响应面优化酶解法制备猪肩胛骨抗氧化肽工艺

Study on the Optimization of Enzymatic Hydrolysis Conditions for Antioxidant Peptide From Porcine Scapula Protein by Response Surface Methodology

为有效利用废弃猪骨制备抗氧化肽,以猪肩胛骨蛋白为原料,采用响应面分析法优化酶解猪肩胛骨蛋白制备抗氧化肽的工艺条件。结果表明,以多肽得率和DPPH自由基清除率为评价指标,与未加酶处理组进行对比,筛选出风味蛋白酶为最优酶。在单因素试验的基础上,根据Box-Behnken中心组合试验设计原理,最终确立最优的酶解工艺参数为酶解时间4 h、酶解温度55℃、酶底比6 200 U·g^(-1)、底物浓度3%、pH值6.0。在此条件下DPPH自由基清除率为75.90%,测得的多肽得率为62.80%,其中R_(adj)~2=97.05%,R2=98.95%,表明该模型拟合程度较好。本研究结果对响应面优化酶解法制备猪肩胛骨抗氧化肽工艺具有一定的实践参考意义。

In order to utilize the porcine scapular to obtain the antioxidant peptide more effectively , the parameters of enzymatic hydrolysis of porcine scapular protein were optimized using response surface analysis. The results showed that the flavourzyme was the best enzyme for the protin hydrolysis by comparing the DPPH scavenging ability and peptide yield. Box-behnken center-united experimental design were carried out based on single-factor experiments. The optimal parameters of hydrolysis technology were 4 h of hydrolysis time, 55℃ of hydrolysis temperature, 6 200 U·g^- 1 of enzyme- substrate ratio, 3% of substrate concentration, and pH value 6.0. Under these conditions, the DPPH seavenging activity of the obtained peptide was 75.90%. Meanwhile, peptide yield was 62. 80% , RAdj^2 =97.05% , R^2 =98.95% , suggesting that the model proposed by this study was acceptable. The results of this study provide a reference for practicaluse of porcine scapula protein to obtain antioxidant peptide by enzymatic hydrolysis.