晚香玉花香成分时空动态变化的研究

Spatial and Temporal Changes of Aromatic Constituents from Polianthes tuberosa

为了探讨晚香玉花香成分的时空动态变化,采用顶空—固相微萃取与气相色谱—质谱联用技术,对晚香玉单瓣和重瓣品种4个花期及单瓣品种花器官不同部位释放的香气成分进行了分析。结果表明,晚香玉不同花期的芳香成分种类和相对含量存在明显差异,单瓣品种花蕾期至盛开期分别检出芳香成分9、13、16和31种,随着花的不断发育,萜烯类化合物含量呈现高-低的变化趋势,醇类化合物含量呈现低-高-低的变化趋势,酚类化合物和酯类化合物含量均呈现低-高的变化趋势;重瓣品种花蕾期至盛开期分别检出芳香成分3、3、8和8种,随着开花的进程推进,萜烯类化合物和酚类化合物含量呈现低-高-低的变化趋势,酯类化合物含量呈现低-高的变化趋势,醇类化合物含量则呈现高-低-高的变化趋势。不同部位释放的香气成分种类和相对含量不同,从晚香玉单瓣品种花瓣、雌蕊、雄蕊中各检测出21、12和20种香气成分,且雌蕊主要释放萜烯类化合物,花瓣和雄蕊主要释放萜烯类和酚类化合物。本研究结果为进一步阐明花香化合物的分子调控机制奠定了基础。

The floral fragrance emitted at four stages of flowering and different flower parts of Polianthes tuberosa were investigated using headspace-solid phase microextraction combining gas chromatography-mass spectrometry. The results showed that the aroma constituents and contents were distinctly different in different flowering stages. In four stages of Polianthes tuberosa cultivar Albino, 9, 13, 16 and 31 species of fragrant component were detected, respectively. Along with the continuous development of flower, terpene compounds showed a high-low pattern, alcohol compounds showed a high-low-high pattern, phenolic compounds and ester compounds showed a trend of low-to-high. As for Polianthes tuberosa cultivar Tall double, 3, 3, 8 and 8 species of fragrant component were determined in four stages respectively. Along with the continuous development of flower, terpene compounds and phenolic compounds followed a pattern of low- high-low, and ester compounds showed a trend of low-high, while alcohol compounds presented a pattern of high-low- high. The aroma constituents and contents were different in different flower organs, 21, 12 and 20 aromatic components were detected from petals, pistil and stamens of Polianthes tuberosa cultivar Albino, respectively. Pistil mainly released terpene compounds, while petals and stamens mainly produced terpene compounds and phenolic compounds. These results provide a strong foundation for further study on molecular regulation mechanism of aroma compounds.