硬叶兜兰菌根真菌的分类鉴定

Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum

以硬叶兜兰菌根真菌为研究对象,采用传统形态学结合rDNA-ITS区的PCR扩增、测序,采用NCBI BLAST在线软件对ITS区序列进行了分析,对硬叶兜兰菌根真菌进行分类鉴定。结果表明:对29株菌根真菌进行rDNA-ITS序列分析共鉴定出23个属,为瘤菌根菌属、胶膜菌属、丝核菌属,镰刀菌属、栓菌属、附毛菌属、拟迷孔菌属、小脆柄菇属、拟层孔菌属、毛壳属、侧耳属、轮枝菌属、弯孢聚壳属等,分离菌株最多的是瘤菌根菌属和胶膜菌属,分别占分离总数的16%和19%;鉴定到1个科,为胶膜菌科;12株优势菌根具有不同的宏观和微观形态,其均未有分生孢子,菌丝分枝点附近形成一个隔及细胞双核,11株菌株具念珠状细胞,大多数具有香味。

Classification and identification of mycorrhizal were associated with Paphiopedilum micranthum,the method of morphological was combined with the ITS gene fragments were PCR amplified and sequenced,the rDNA ITS regions were analyzed by means of the software of NCBI BLAST.The results showed that the 29 strains with different micro-and macro-morphological.Secondly,rDNA-ITS sequence analysis of 29 predo minant strains.The fungi were identified into 23 genus and 1 family,23 strains belong to Epulorhiza,Tulasnella,Rhizoctonia,Fusarium,Trametes,Trichaptum, Daedaleopsis, Psathyrella, Fomitopsi, Chaetomium, Pleurotus, Lecanicillium,Eutypella,Lecanicillium,Peniophorella,which their frequency were 16% and 19%.Only one family belonged to Tulasnellaceae.12 strains of dominant mycorrhiza had different microcosmic and macroscopic,none of them had conidiophore,near the hyphal branching-points formed of septum and conjugate nuclei,11 strains mycorrhiza with intestiniform cells,the most of mycorrhiza had a distinct aroma.