DAP12在肝细胞癌组织中的表达及临床意义

Expression of DAP12 in Hepatocellular Carcinoma and Its Clinical Significance

目的:研究DAP12在肝细胞癌(HCC)组织中的表达并分析其与患者临床病理特征及预后的关系。方法:收集94例手术切除的HCC标本,采用免疫组织化学染色方法检测其DAP12、CD163的表达情况,免疫荧光共定位检测二者在HCC组织中表达的相关性。单因素统计分析DAP12与临床病理特征的关系,进一步以多因素Cox比例风险回归分析影响患者生存时间的危险因素。结果:DAP12和CD163主要表达于HCC组织内的间质细胞,且DAP12和CD163的表达位置重叠,二者表达呈显著正相关性(r=0.775,P=0.001)。DAP12表达与肿瘤直径、病理组织分级及TNM分期有关(P=0.007、0.036、0.013)。DAP12高表达组患者生存时间显著短于低表达组患者(x2=12.018,P=0.005)。DAP12高表达、TNM分期是影响患者预后的独立危险因素(P=0.013,0.039)。结论:DAP12主要在HCC组织内替代活化型巨噬细胞(TAMs)中表达,DAP12阳性TAMs浸润密度与HCC的恶性进展及预后密切相关。

Objective： To investigate the expression of DAP12 in the hepatocellular carcinoma （HCC） and the correlation with the clinicopathological parameters and prognosis of HCC patients. Methods： 94 cases of HCC specimens were collected to detect the expres- sion of CD163 and DAP12 and the correlation of DAP12 and CD163 expression in HCC tissues were analyzed via immunohistochemical staining and colocalization of immunofluorescence, respectively. The single factor statistical analysis was performed to verify the rele- vance between DAP 12 expression and clinicopathological factors. The multivariate Cox proportional hazard regression analysis was car- fled out to find out the involving factors impacting the survival time of HCC patients. Results： The expression of DAP12 and CD163 were tested in HCC interstitial cells and overlapped, which were positively correlated （r=0.775, P〈0.001）. DAP12 was proved to contri- bute to tumor diameter, Histological grade and TNM stage via single factor analysis （P=0.007, 0.036, 0.013）. The survival time of pa- tients with high DAP 12 expression was significantly shorter（P=0.005）. Multivariate Cox proportional hazards model analysis showed that high DAP12 expression and TNM stage were independent risk factors affecting the prognosis of patients, respectively （P =0.013, 0.039）. Conclusion： DAP12 mainly expressed in alternatively activated macrophages in HCC tissues. Meanwhile, DAPI2 positive TAMs infil- trated density in HCC tissue was closely related to the malignant progression and prognosis of HCC.