肥胖动脉粥样硬化大鼠模型的建立及验证

A Rat Model of Dietary Induced Atherosclerosis and Obesity

目的:通过饮食及药物诱导营养性肥胖及动脉粥样硬化大鼠模型的建立及评价。方法:健康成年wistar大鼠随机分组,利用钙负荷联合高脂饮食的方法建立肥胖动脉粥样硬化大鼠模型;在模型建立不同时期分别检测各组实验大鼠身长、体重及腹围情况,并测量不同阶段大鼠皮下脂肪、腹腔内脏脂肪及胸主动脉血管旁脂肪组织质量;测量大鼠血清中甘油三酯、总胆固醇、低密度脂蛋白胆固醇及高密度脂蛋白胆固醇浓度变化;测量大鼠各部位脂肪含量变化;病理HE染色明确动脉粥样硬化形成状况。结果:模型各组大鼠血清总胆固醇及低密度脂蛋白胆固醇明显增高;病理HE染色证明模型组大鼠造模8周即可形成动脉硬化表现,并且伴随造模时间的延长,胸主动脉中膜厚度明显增加;模型组大鼠在造模16周开始体重、腹围、身体各部位脂肪组织含量较对照组明显增加,肥胖模型成功。结论:于研究16-24周时间成功构建了肥胖及动脉粥样硬化共病大鼠模型,为进一步研究肥胖及动脉粥样硬化的发生发展机制提供了可靠的动物模型。

Objective： This research aims at establishing rats model of obesity and atherosclerosis. Methods： Healthy adult male wistar rats were randomly assigned to model group and normal control group. The model group rats were orally fed with vitamin D3 and high fat diet. Measure body length, body weight and abdominal circumference in different stages. Harvest subcutaneous adipose tissue, visceral adipose tissue and perivascular adipose tissue of thoracic aorta and measure weight of different fat depots.Serum total cholesterol （TC）, total triglyceride （TG）, LDL-cholesterol （LDL-c） and HDL-cholesterol （HDL-c） were measured. Hematoxylin-eosin（HE） staining was used to detect the condition of aortic artery atherosclerosis, and measure the thickness of aorta wall. Results： Serum total cholesterol and LDL-cholesterol of model group rats increased significantly compared with the normal cona＇ol group rats; HE staining prove the atherosclerosis model was successfully established after eight weeks by feeding with high fat diet. Thoracic aortic media thickness was significantly increased. The body weight, abdominal circumference and adipose tissue weight of model group rats significantly increased after sixteen weeks. Conclusions： After 16-24 weeks, a rat model of obesity and atherosclerosis was successfully got, which provided a reliable animal model for further study on the mechanism of obesity and atherosclerosis.