全身稳恒磁场暴露对糖尿病性动脉粥样硬化大鼠血脂和血液流变学的影响

Effect of Whole-body Exposure to Static Magnetic Fields on the Blood Lipids and Hemorheology of Diabetic Atherosclerosis Rats

目的:探索全身暴露强度为4.0 m T中强度稳恒磁场对于糖尿病性动脉粥样硬化的血脂和血液流变学影响,以明确稳恒磁场对糖尿病性动脉粥样硬化的潜在作用效果。方法:选择3月龄雄性SD大鼠30只,随机等分至空白对照组、糖尿病组及糖尿病磁场暴露组。糖尿病和糖尿病磁场暴露组的大鼠采用链脲佐菌素+维生素D3+高脂饮食组合作用法建立糖尿病性动脉粥样硬化大鼠模型,对糖尿病磁场暴露组施加2小时/天、强度为4.0 m T的全身稳恒磁场暴露,连续刺激8周后,检测空腹血糖、血清胰岛素、血脂四项(血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇以及低密度脂蛋白胆固醇)以及血液流变参数(低切全血粘度、中切全血粘度、高切全血粘度以及血浆粘度)。结果:稳恒磁场暴露显著抑制了糖尿病动脉粥样硬化大鼠血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇的升高(P<0.05),同时也显著降低了低切、中切和高切全血粘度以及血浆粘度(P<0.05),但对血糖和血清胰岛素的影响不显著(P>0.05)。结论:中强度稳恒磁场可显著降低糖尿病性动脉粥样硬化大鼠的血脂水平并改善其血液流变学。

Objective： To explore the effects of whole-body exposure to moderate-intensity static magnetic fields on the blood lipids and hemorheology of diabetic atheroselerosis rats, so as to identify the potential regulator effects of static magnetic fields on diabetic atherosclerosis. Methods： Three-month-old male SD rats （n=30） were randomly and equally assigned into the blank control （Control）, diabetes mellitus （DM） and diabetes mellitus with static magnetic fields exposure （DM＋SMF） groups. The rats in the DM and DM＋SMF groups were subjected to the combined administration with streptozotocin, vitamin D3 and high-fat diet to induce diabetic atherosclerosis animal model. The rats in the DM＋SMF group were stimulated with 2 hours/day whole-body static magnetic fields at 4.0 mT intensity for 8 weeks. Then, the blood samples were collected, and blood lipid indices （serum total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol） and hemorheology indices （low-shear blood viscosity, medium-shear blood viscosity, high-shear blood viscosity and plasma viscosity） were determined. Results： Static magnetic fields significantly inhibited the increase of serum total cholesterol, triglyceride, high-density lipoprotein cholesterol and low,density lipoprotein cholesterol in diabetic atherosclero- sis rats （P〈0.05）, and also significantly increased low-shear blood viscosity, medium-shear blood viscosity, high-shear blood viscosity and plasma viscosity （P〈0.05）. Nonetheless, static magnetic fields exhibited no obvious effects on glucose and serum insulin in diabetic atherosclerosis rats （P〉0.05）. Conclusion： Moderate-intensity static magnetic fields exhibited positive therapeutic effects on the blood lipids and hemorheology of diabetic atherosclerosis.