盐酸法舒地尔对大鼠脑缺血-再灌注损伤的保护作用

The neuroprotective effects of Fasudilon cerebral ischemia-reperfusion injury on rats

目的 :验证一种新药盐酸法舒地尔 (Fasudil)作为细胞内钙离子拮抗剂对大鼠缺血再灌注损伤的保护作用。方法 :用线栓法和转流法制作大鼠大脑中动脉区缺血再灌注模型 ,于缺血前 30min分别给予盐酸法舒地尔和生理盐水。观察下列指标 :缺血后 5min、60min和再灌注 30min的局部脑血流量 (rCBF) ;术后 3h、2 4h及 48h神经功能 ;缺血 60min、再灌注 2 0min、60min、1 2 0min缺血脑组织乳酸含量。结果 :法舒地尔组局部脑血流(rCBF)明显高于对照组 ;法舒地尔组神经功能好于对照组 ;再灌注 60min、1 2 0min乳酸含量法舒地尔组少于对照组。结论 :盐酸法舒地尔能改善动物模型缺血脑组织局部血流量 ,加速再灌注过程乳酸清除 。

Objective:Investigate the neuroprotective effects of Fasudil on cerebral ischemia reperfusion injury Methods: Animal models of reformed middle cerebral artery occlusion were made in 80 SD rats, treated by Fasudil or normal saline 30 minutes before the ischemia event, then recorded the regional blood flow of the ischemia tissue 5min, 60min after ischemia, and 30min after reperfusion ; neurological outcome after 3 hour, 24 hour, and 48 hour periods; the amount of lactic acid in ischemia area 60min after ischimia, 20, 60 and 120 minutes after reperfusion Results: The rCBF was higher in the Fasudil group than in the control group; the tissue content of lactic acid was less in the Fasudil group 60 and 120 min after reperfusion Conclusion: Fasudil increases rCBF of the ischemia area, accelerates removing of lactic acid, effectively protects brain tissue