补肾活血通腑方对慢性脑供血不足模型大鼠SIRT1信号通路下蛋白表达的影响

Effect of Bushen Huoxue Tongfu Formula on Protein Expression in SIRT1 Signaling Pathway in Rat Model of Chronic Cerebral Blood Deficiency

目的:探讨补肾活血通腑方对慢性脑供血不足(CCCI)大鼠脑组织的保护作用。方法:雄性健康Wistar大鼠50只,随机分为五组:假手术组、模型组、尼莫地平组、补肾活血通腑方低剂量组及高剂量组,采用2-VO永久结扎剪断方法制备CCCI大鼠模型。大鼠连续给药灌胃8周之后,断头取海马,运用Western blot法进行蛋白检测。结果:与假手术组相比,模型组和其他治疗组在额叶PGC-1α及FOXO3α蛋白表达明显下降(P<0.05)。与模型组相比,补肾活血通腑方低剂量组海马的PGC-1α蛋白表达明显增加(P<0.05);与尼莫地平组相比,补肾活血通腑方低剂量组FOXO3α、PGC-1α蛋白表达明显增加(P<0.05)。结论:CCCI大鼠的脑缺血可以抑制FOXO3α、PGC-1α蛋白表达;补肾活血通腑方低剂量对FOXO3α、PGC-1α蛋白的表达有改善作用,增加脑供血,对CCCI大鼠脑组织有保护作用。

Objective：To study the protective effect of Bushen Huoxue Tongfu formula（BHTF） on brain tissue of rats with chronic cerebral circulatory insufficiency （CCCI）. Methods：Fifty healthy male Wistar rats were randomly divided into five groups：sham operation group,model group,nimodipine group,BHTF of low dose group,BHTF of high dose group. The CCCI rat model was established using 2-VO permanent ligation. After 8 weeks of intragastric administration,the rats were sacrificed through decapitation,and the hippocampus was harvested. The protein was detected by Western blot. Results：Compared with the sham group,the expression of PGC-1α and FOXO3α in the frontal lobe of the model group and other treatment groups were decreased significantly（P〈0.05）. Compared with the model group,the expression of PGC-1α protein in the hippocampus of the BHTF of low dose group was significantly higher than that in the model group （P〈0.05）. Compared with the nimodipine group,FOXO3α,PGC-1α protein expressions were significantly increased （P〈0.05）. Conclusion：Cerebral ischemia in CCCI rats can inhibit the expressions of FOXO3α and PGC-1α proteins. BHTF of low dose can improve the expressions of FOXO3α and PGC-1α proteins. Increase the blood supply of the brain,thereby protecting the brain tissue of CCCI rats.