HPLC测定苏木配伍马钱子前后对巴西苏木素和原苏木素B含量的影响

Determine Changes of Brazilin and Protosappanin B after Lignum Sappan Combined with Semen Strychni Using HPLC

目的:测定巴西苏木素和原苏木素B在马钱子配伍苏木(马苏)不同比例水煎剂中的含量变化。方法:制备马苏煎液1∶6组、1∶8组、1∶12组以及1∶24组,采用高效液相色谱法(HPLC)检测巴西苏木素和原苏木素B含量的变化。色谱柱为Agilent SBC18(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%醋酸,梯度洗脱,流速为1.0 mL/min,检测波长为285 nm。结果 :经检测马苏煎液中两种化学成分的线性范围保持在0.025~1.50 mg/mL,两者的相对保留值是0.9997。结论:证明了不同比例的马苏水煎液中巴西苏木素与原苏木素B的含量均保持在梯度式升高的趋势上。

Objective：To determine the content change of brazilin and protosappanin B in the combination of Semen Strychni and Lignum Sappan with different proportion in the water decoction. Methods：The water decoction was of different proportion at 1：6,1：8,1：12 and 1：24. High performance liquid chromatography （HPLC） was used to determine the content change of brazilin and protosappanin B. The column was Agilent SBC18（250 mm× 4.6 mm,5 μm）,the flow phase was methanol-acetic acid（0.1% ）,the flow velocity was 1.0 mL/ min, and the detection wavelength was 285 nm. Results ：The linearity range of brazilin and protosappanin B was from 0.025 mg/ml to 1.50mg/mL,and the relative retention was 0.9997. Conclusion：The experimental resuits show that in different proportion of water decoction of Semen Strychni and Lignum Sappan,the contents of brazilin and protosappanin B tend to increase in gradient.