人离体子宫内膜细胞培养方法的建立

Isolated cell cultures of human endometrium in vitro

目的 :建立分离培养人在位及异位子宫内膜间质及腺上皮细胞的方法 ,为进一步探讨子宫内膜异位症发生 ,发展的分子生物学机制提供一个理想的实验模型。方法 :对 36例在位子宫内膜及 1 9例异位内膜随机分为离心分离组及筛网分离组进行体外培养。通过光镜观察及S P免疫组化染色法 ,对间质及上皮细胞进行鉴定。结果 :离心分离法培养的在位和异位内膜细胞中 ,成功率分别为 61 1 %和 30 % ;筛网分离法培养的在位和异位内膜细胞中 ,成功率分别为 83 3 %和 77 8%。结论 :采用筛网分离法及离心分离法均能获得纯度较高的间质与上皮细胞。人子宫内膜细胞分离体外培养的成功 ,有助于揭示子宫内膜异位症的发病机制 ,为临床治疗提供重要的理论依据。

Objective:To establish highly purified cultures of epithelial and stromal of endometrium in vitro Methods: thirty six samples of eutopic endometrial tissues and nineteen samples of ectopic endometrium were separated by filter and centrifugation method The cells were detected through micoscopy and immunohistochemical experiments Results: The success cultured rate of endometrium and endometriotic of filter isolated method were 61 1% and 30%, respectively And that of centrifugation method were 88 3% and 77 8%, respectively The survival periods of epithelial cells were shorter than stromal cells Conclusion: It is conclude that highly purified epithelial and stromal cells cultured from normal endometrial and endometriotic tissues were obtained by filter and centrifugation isolated cell culture, but filter isolated method is better than centrifugation method The cellular model will be useful for us to investigate the factors that might influence or promote the degree of invasiveness of endometriotic cells and contribute to its pathogenesis