白细胞介素-17A及其受体在前列腺癌中的表达及意义

Expression and significance of IL-17A,IL-17RA in prostate cancer

目的探讨白细胞介素(IL)-17A及其受体IL-17RA在前列腺癌(Pca)组织中的表达及意义。方法应用免疫组织化学染色法分别检测6例正常前列腺(NP)、39例良性前列腺增生(BPH)和20例Pca标本中IL-17A、IL-17RA表达情况;炎症细胞(淋巴细胞、巨噬细胞、中性粒细胞和肥大细胞)浸润情况。结果 IL-17A主要表达于腺上皮,单个核细胞和血管内皮细胞,也可见部分癌细胞内,在NP、BPH及Pca阳性表达率分别为为3.77%±0.04%、5.58%±0.15%、8.82%±0.06%,Pca与BPH和NP比较有明显差异(P<0.05);IL-17RA主要表达于腺上皮,单个核细胞和血管内皮细胞,也可见部分癌细胞,在NP,BPH及Pca表达率阳性分别为3.36%±0.20%、4.12%±0.17%、8.29%±0.21%,Pca与BPH和NP比较表达明显增加(P<0.05)。淋巴细胞在NP,BPH和Pca中的阳性表达密度分别为(1.40±0.01)个/mm^2、(4.11±0.03)个/mm^2、(4.12±0.05)个/mm^2,Pca和BPH与NP比较差异明显(P<0.05);巨噬细胞在NP,BPH和Pca的阳性表达密度分别为(1.25±0.13)、(6.82±0.04)、(4.71±0.02)个/mm^2,Pca和PH与NP比较差异明显(P<0.05);肥大细胞在NP,BPH和Pca中的阳性表达密度分别为(1.01±0.03)、(7.21±0.01)、(7.35±0.01)个/mm^2,Pca和BPH与NP比较差异明显(P<0.05);中性粒细胞在NP,BPH和Pca的阳性表达密度分别为(0.72±0.01)、(4.73±0.08)、(4.42±0.01)个/mm^2,Pca与BPH和NP比较差异明显(P<0.05)。BPH组织中IL-17A阳性表达与IL-17RA呈正相关(r=0.368,P=0.025);Pca组织中IL-17RA阳性表达与肥大细胞阳性密度呈正相关(r=0.523,P=0.021)。结论 IL-17A与IL-17RA在Pca中表达明显增加,IL-17RA可能主要由肥大细胞分泌,与Pca的发病密切相关;而IL-17A与IL-17RA的信号转导在BPH发生发展中起重要作用。

Objective To explore the expression and significance of IL-17 A and IL-17RA in prostate cancer（ Pca）. Methods Immunohistochemistry staining was applied to detect IL-17 A,IL-17RA and related inflammatory cells in 6 cases of normal prostate（ NP） specimens,39 cases of BPH specimens and 20 cases of Pca specimens. Results IL-17A mainly expressed glandular epithelium,mononuclear cells and vascular endothelial cells as well as partial cancerous cells. In NP,BPH and Pca its positive expression percentage was 3. 77% ±0. 04%,5. 58% ±0. 15% and 8. 82% ±0. 06% respectively. Compared with NP and BPH it had obviously increased in Pca（ P〈0. 05）. IL-17RA also mainly expressed in glandular epithelium,mononuclear cells,vascular endothelial cells and partial cancer cells. In NP,BPH and Pca its positive expression percentage was 3. 36% ±0. 20%,4. 12% ±0. 17%,8. 29±0. 21%. Compared with NP and BPH,there was obvious statistical difference in Pca（ P〈0. 05）. Lymphocyte distribution density in NP,BPH and Pca was（ 1. 40±0. 01） mm^-2,（ 4. 11±0. 03） mm^-2 and（ 4. 12±0. 05） mm^-2 respectively,there existed obvious increase in Pca and BPH（ P〈0. 05）,while there was no difference between Pca and BPH. Macrophages distribution density in NP,BPH and Pca was（ 1. 25±0. 13） mm^-2,（ 6. 82±0. 04） mm^-2 and（ 4. 71±0. 02） mm^-2. In BPH and Pca they increased obviously（ P〈0. 05）. Mast cells distribution density in NP,BPH and Pca was（ 1. 01 ± 0. 03） mm^-2,（ 7. 21±0. 01） mm^-2 and（ 7. 35±0. 01） mm^-2 respectively. In BPH and Pca they accumulated obviously（ P〈0. 05）. Neutrophils distribution density in NP,BPH and Pca was（ 0. 72±0. 01） mm^-2,（ 4. 73±0. 08） mm^-2 and（ 4. 42±0. 01） mm^-2 separately. In Pca it elevated obviously compared with NP and Pca while between BPH and NP there was no obvious difference（ P〈0. 05）. In BPH there existed obvious positive correlation between IL-17 A and IL-17RA（ r = 0. 368,P = 0. 025）. In Pca obvious positive correlation was found between IL-17RA and mast cells（ r =0. 523,P = 0. 021）. Conclusions Both IL-17 A and IL-17RA expression are increased significantly in Pca and IL-17RA mainly is secreted by mast cells,which is related with prognosis of Pca. IL-17A and IL-17RA participate in BPH occurrence and development. Both IL-17A and IL-17RA participate in the occurrence and development in BPH and Pca.