灯盏花素对原代培养的牛肺动脉平滑肌细胞增殖的抑制作用

Inhibitory effect of breviscapine on primary cultural calf pulmonary artery smooth muscle cell proliferotion

目的 研究灯盏花素对肺动脉平滑肌细胞增殖的影响。方法 用原代培养的小牛肺动脉平滑肌细胞 ,采用MTT比色法、3 H TdR参入法、流式细胞术、Giemsa染色对肺动脉平滑肌细胞增殖的影响进行观察。结果 与对照组相比 ,灯盏花素处理组可抑制肺动脉平滑肌细胞的增殖 ,而且主要是细胞周期的G0 /G1到S期受机制。结论 灯盏花素可显著地抑制肺动脉平滑肌细胞的增殖 。

AIM To explore the effect of breviscapine on primary cultural calf pulmonary artery smooth muscle cell (PASMC) proliferation. METHODS By primary cuture of calf PASMC, the effect of PASMC proliferation was analysed using MTT colorimetry, flow cytomerty, Giemsa staining. RESULTS Compared with control group, PASMC proliferations in breviscapine group was significantly inhibited from G 0/G 1 to S phases in the cell cycle. CONCLUSION Breviscapine could significantly inhibit PASMC profiferation. The mechanism for this may due to inhibition of protein kinase C.