朱砂莲提取物(A_(1015))对D-氨基半乳糖胺肝损伤模型小鼠DNA合成作用的影响

The effect of A_(1015) on DNA synthesis of D-galactosamine damaged mice hepatotoxicity

目的 研究朱砂莲提取物 (A10 15)对D 氨基半乳糖胺(D Gl)肝损伤模型小鼠DNA合成作用的影响。方法 采用D Gl造模 ,3 H TdR参入法测定小鼠肝细胞DNA合成 ,并与肝细胞再生因子进行比较。观察了A10 15的剂量曲线和时间曲线。结果和结论 朱砂莲提取物 (A10 15)抵抗D Gl造成的肝组织坏死和促进肝脏细胞DNA合成作用的最适剂量为 2 5mg·kg-1体重。试验还提示A10 15的抗肝中毒作用2 0 0 1-12 -2 6收稿 ,2 0 0 2 -0 3 -0 6修回1 华西医科大学基础部药理学教研室 ,成都 610 0 41作者简介 :刘碧崇 ,女 ,3 7岁 ,副研究员 ,博士。研究方向 :微生物药物与中药研究。Tel:0 2 8 43 780 40 ,Fax :0 2 8 43 3 3 2 18,E mail:huanggen @mail sc cninfo net;王浴生 ,男 ,81岁 ,教授 ,博士生导师。研究方向

AIM To study the effect of A 1015 on D galactosamine induced mice hepatotoxicity. METHODS The effect of three doses of A 1015 on DNA biosynthesis of D galactosamine induced mice hepatotoxicity were tested. The time curve of A 1015 on DNA synthesis of D GL induced mice hepatotoxicity was also observed. RESULTS The experiments demonstrated that 2 5 mg·kg -1 is the optimum dose for A 1015 to show liver protective activity. The results suggested that HGF can not only offset the hepatotoxicity induced by D GL, but also promote the DNA synthesis of mice liver cells. And the highest DPM value for HGF group is higher than that of A 1015 group. Thus both HGF and A 1015 showed anti hepatotoxic activity and promoting activity on mice liver cell DNA synthesis. CONCLUSION A 1015 may reduce liver necrosis induced by D galactosamine and promote the DNA synthesis of mice liver cells.