黄莲提取液对人神经胶质瘤细胞体外增殖的影响及其作用机制

Effect of goldthread root extract on proliferation in vitro of human glioma cells and relevant mechanism

目的探讨黄莲提取液对人神经胶质瘤细胞(C6)体外增殖的影响及其可能的作用机制。方法用10、20、40、60μl/ml的黄连提取液分别作用C6细胞48 h后,倒置显微镜下观察C6细胞形态;作用24、48、72 h后,MTT法检测黄莲提取液对C6细胞体外增殖的影响。用40μl/ml的黄连提取液分别作用C6细胞0、20、40及60 min后,Western blot法分析C6细胞中Bcl-2和Bax蛋白的表达水平。结果倒置显微镜下观察可见,随着黄莲提取液浓度的升高,坏死细胞的数量逐渐增多;40μl/ml黄莲提取液作用48 h时的抑制率显著高于10及20μl/ml浓度组(P<0.05),与60μl/ml浓度组差异无统计学意义(P>0.05);C6细胞中Bax蛋白表达水平随黄莲提取液作用时间的延长而上升(P<0.05),Bcl-2蛋白表达水平随黄莲提取液作用时间的延长而下降(P<0.05)。结论黄莲提取液对C6细胞的增殖具有显著的抑制作用,且可能是通过Bcl-2途径实现的。

Objective To investigate the effect of goldthread root extract on proliferation in vitro of human glioma cells as well as the possible mechanism. Methods C6 cells were treated with 10, 20, 40 and 60 μl/ml goldthread root extract for48 h, and observed for morphology by microscopy. The proliferation levels in vitro of C6 cells 24, 48 and 72 h after treatment were determined by MTT assay. C6 cells were treated with 40 μl/ml goldthread root extract for 0, 20, 40 and 60 min, and determined for expression levels of Bcl-2 and Bax proteins by Western blot. Results The necrotic cells increased with the increasing concentration of goldthread root extract. The inhibition rate of proliferation of cells 48 h after treatment with40 μl/ml goldthread root extract was significantly higher than those with 10 and 20 μl/ml（P 0. 05）, which showed no significant difference with that with 60 μl/ml（P 0. 05）. The expression level of Bax protein in C6 cells increased, while that of Bcl-2 protein decreased, with the time for treatment with goldthread root extract（each P 0. 05）. Conclusion Goldthread root extract showed significantly inhibitory effect on proliferation of C6 cells by a possible Bcl-2 pathway.