杉木不完整型EST-SSR特征分析

Characteristic Analysis of Incomplete EST-SSR in Cunninghamia lanceolata

SSR可以分为完整型(不间断单一型)和不完整型(包含不间断复合型、间断单一型、间断复合型)。本研究旨在分析杉木(Cunninghamia lanceolata)不完整型EST-SSR特征。结果表明:转录组搜索出5 999个不间断单一型EST-SSR,占全部EST-SSR的97.78%,不完整型所包含3种类型EST-SSR的数量接近,SSR数量之和为136个,仅占转录组全部EST-SSR的2.22%。在杉木136个不完整型EST-SSR中,共有单核苷酸、二核苷酸、三核苷酸、四核苷酸、六核苷酸等5种基元重复类型。不间断复合型EST-SSR中基元重复类型的组合数最多,出现了7种组合,间断复合型出现5种组合,间断单一型出现3种组合。

SSRs （Simple sequence repeats） could be divided into perfect repeats （simple repeats without interruption） and imperfect repeats （including compound repeats without interruption, simple repeats with in- terruption, compound repeats with interruption）. The characteristics of incomplete EST-SSR in Cunning- hamia lanceolata were analyzed. The results showed that without interruption） EST-SSRs in the transcriptome, there were 5 999 perfect （simple repeat sequences which accounted for 97.78% of all EST-SSRs. The number of three types of imperfect EST-SSRs was similar to each other. All these three types of SSRs added up to 136, accounting for only 2.22% of all EST-SSRs. Among the 136 imperfect EST-SSRs in C. lanceolata, there were 5 kinds of motif repeat types in total, such as mononucleotide, dinucleotide, trinucleotide, tetranucleo- tide, and hexanucleotide. Among the compound EST-SSRs without interruption, there were 7 combinations of element repeats, which was the largest number of combinations. While in compound EST-SSRs with interruptions, there were 5 combinations, and in simple EST-SSRs with interruptions, there were 3 combinations.