对山东地区TTV基因片段的检测及其部分核酸序列测定

Partial gene of transfusion transmitted virus (TTV) detecting and sequencing in Shandong

目的 了解山东地区输血传播病毒 (TTV)分离株感染状况及基因变异的情况 .方法 应用逆转录—聚合酶链反应法 (RT- PCR)检测山东地区 2 6例非甲～戊型肝炎和 1 2例肝细胞癌患者血清中的 TTV DNA,对阳性扩增的产物进行测序 ,并分析其基因变异情况 .结果 2 6例非甲～戊型肝炎患者检出 TTV DNA阳性者 1 1例 (42 % ) .对其中 2例(TTVSD4,TTVSD5)进行 PCR产物直接测序 ,并与日本株(AB0 0 8394)相比较 ,其核苷酸序列同源性分别为 99.9%和1 0 0 % .而 1 2例肝细胞癌患者中 TTVDNA阳性 3例 (2 5% ) ,对其中 1例 (TTVSD6)测序 ,与日本株 (AB0 0 8394)相比较 ,其核苷酸序列同源性为 99% ;TTVSD4、 TTVSD5和TTVSD6之间的核苷酸同源性均为 99% .结论 山东地区非甲～戊型肝炎和肝细胞癌患者中 TTV感染率较高 ,测序结果表明其与日本株 (AB0 0 8394)

AIM To investigate the prevalence of TTV infection and genetic variation of TTV isolate in Shandong Province. METHODS TTV DNA were amplified and detected by RT PCR methods from sera of patients with nonA E hepatitis ( n =26) and hepatocellular carcinoma ( n =12). The positive PCR products were sequenced and analyzed for genetic variation. RESULTS TTV DNA were detectable in 11 of 26 patients (42.3%) with nonA E hepatitis. Two of them (TTVSD4, TTVSD5) were sequenced and compared with known sequence of TTV isolates in Japan (AB008394) . The nucleotide homology was 99% and 100%. TTV DNA was positive in 3 of 12 patients (25%) with hepatocellular carcinoma. One of them (TTVSD6) was sequenced and compared with AB008394. The nucleotide homology was 99%. CONCLUSION TTV infection is high in the nonA E hepatitis and hepatocellular carcinoma patients in Shandong province. The secquence results showed that the TTV isolate in Shandong province has high homology with TTV isolate AB008394 in Japan.