脾虚证与胃窦D细胞的关系

Alteration of D cells in gastric antrum in spleen-asthenia syndrome

目的 探讨脾虚证与生长抑素 (somatostain,SS)内分泌细胞 (D细胞 )数量变化的关系 .方法 用免疫组化链酶亲合素过氧化酶复合技术 (SABC)对利血平脾虚大鼠模型胃粘膜和患者胃粘膜组织的 D细胞染色 ;观察各组 D细胞的形态和数量变化 .结果 和对照组相比 ,脾虚大鼠模型 7d组 D细胞数升高 (43.4± 3.1 vs78.6± 4.6,P<0 .0 1 ) ,强阳性细胞率下降 [(34.6± 2 .5) % vs(2 4 .1± 3.5) % ,P<0 .0 1 ] ;脾虚大鼠模型 1 4 d组 D细胞强阳性细胞率升高 [(34.6± 2 .5) % vs(46.4± 9.0 ) % ,P<0 .0 5] ;四君子汤治疗组大鼠与正常对照组间 D细胞数 (43.4± 3.1 vs41 .7± 3.7)及强阳性细胞率无显著差异 [(34.6± 2 .5) % vs(33.1± 1 .7) % ,P>0 .0 5] .患者脾虚组 D细胞阳性数 (70 .4± 1 0 .1 ) ,(38.1± 6.4) ,(45.4± 8.6) ,(P<0 .0 1 )及强阳性率较胃热、肝火上炎组均增加 (43.2± 8.7) % ,(1 7.9± 6.6) % ,(2 1 .8± 7.5) % (P<0 .0 1 ) .结论 脾虚证大鼠和患者胃粘膜组织中 D细胞阳性和强阳性细胞率增加 ,提示脾虚患者临床出现腹胀、纳差等症状可能是由于

AIM To investigate the relationship between somatostain enteroendocrine cells and spleen asthenia syndrome in rats and patients. METHODS Short term and the long term rat models of spleen asthenia syndrome were treated by reserpine for seven days and fourteen days. The patients with spleen asthenia syndrome (12), stomach heat syndrome (11) and flaming up of liver fire syndrome (9) were selected by differential diagnosis and immunohistochemical SABC method was employed to stain the D cells of gastric antrum. The variation of appearance and quantity of endocrine cells were observed by light microscope. RESULTS In the rat model of seven days, as compared with the controls, the number of D cells increased (43.4±3.1), (78.6±4.6) ( P <0.01), and the percentage of strongly positive D cells reduced (34.6± 2.5)%, (24.1±3.5)% ( P <0.01). In the rat model of fourteen days, the percentage of strongly positive D cells increased (34.6±2.5)%, (46.4±9.0)% ( P <0.01), In patients with spleen asthenia syndrome the quantity (70.4±10.1), (38.1±6.4), (45.4±8.6)% and the percentage of strongly positive of D cell (43.2±8.7)%, (17.9±6.6)%, (21.8±7.5)% ( P <0.01) increased as compared with the other groups. CONCLUSION Quantity and the percentage of strongly positive D cells of gastric antrum increased in spleen asthenia syndrome, and different symptoms of spleen asthenia may be related to the increased function of D cells.