增殖细胞核抗原和CD44mRNA在伴有静脉侵袭的结直肠癌中的表达及其与肝转移的关系

Expression of PCNA and CD44 mRNA in venous invasional colorectal cancer and its relationship to li-ver metastasis

目的 研究伴有静脉侵袭的结直肠癌组织中增殖细胞核抗原 (PCNA)和 CD44m RNA的表达及其与肝转移的关系 .方法 采用反转录 -聚合酶链式反应 (RT- PCR)方法检测31例伴有静脉侵袭的结直肠癌组织中 PCNA和 CD44m R-NA的表达状况 .结果 PCNA和 CD44m RNA在结直肠癌组织中阳性表达率分别为 1 0 0 %和 64.5% .在有肝转移的结直肠癌组织中 ,PCNA和 CD44 m RNA的强表达率分别为94.1 %和 70 .6% ,表达强度明显高于无肝转移者 (P<0 .0 5和P<0 .0 1 ) .PCNA和 CD44的表达存在正相关 (r=0 .67,P<0 .0 5) .结论 结直肠癌组织中 PCNA和

AIM To investigate the expression of prolifera ting cell nuclear antigen (PCNA) and CD44 mRNA in venous invasional colorectal cancer and its relationship with liver metastasis. METHODS Reverse transcriptase polymerase chain reaction (RT PCR) was used to detect the expressions of PCNA and CD44 mRNA in 31 cases of colorectal cancer with liver metastasis. Theexpressions of PCNA and CD44 mRNA in 31 cases of venous invasional colorectal cancer. RESULTS Positive expression rates of PCNA and CD44 mRNA in the colorectal cancer were higher than those without liver metastasis ( P <0.05 and P <0.01). In cases of colorectal cancer with liver metastasis, 94.1% and 70.6% were strongly positive to PCNA and CD44 mRNA, respectively, significantly higher than those without liver metastasis. There was a positive relationship between the expressions of PCNA and CD44 ( r=0.67, P <0.05). CONCLUSION Detections of PCNA and CD44 expressions in colorectal cancer might be useful for evaluating the liver metastasis of cancer cells.