SLE患者血中IL-38的水平及其与病情关系的研究

Investigation on relationships between serum level of IL-38 and disease activity in patients with systemic lupus erythematosus

目的:比较系统性红斑狼疮(SLE)患者与健康对照者外周血单个核细胞(PBMCs)中IL-38mRNA表达量及血清IL-38水平的差异,分析血清IL-38水平与主要病情评价指标的关系。方法:用RT-PCR检测30例SLE患者及20例对照者PBMCs中IL-38 mRNA表达量,用ELISA法测定72例SLE患者以及63例对照者血清IL-38水平,比较SLE患者与对照者IL-38的mRNA及血清水平是否具有差异,分析血清IL-38阴性(血清IL-38<31.2 pg/m L)与IL-38阳性(血清IL-38≥31.2 pg/m L)患者主要临床指标的差异。结果:SLE患者IL-38 mRNA表达量、血清IL-38阳性率以及血清IL-38水平均明显低于健康对照者(Z=-2.34,P=0.019;x^2=10.19,P=0.002;Z=-4.04,P<0.001)。血清IL-38阳性的患者抗ds DNA抗体水平均为阴性,且其SLEDAI评分低于IL-38阴性患者(t=-2.76,P=0.020)。结论:SLE患者外周血中IL-38的降低可能参与了SLE的发病及病情活动过程。

Objective：To compare the IL-38 mRNA expression level of peripheral blood mono- nuclear cells （PBMCs） and serum IL-38 levels between systemic lupus erythematosus （SLE） patients and healthy controls, and to analyze the relationship between serum IL-38 level and major clinical data of SLE patients. Methods： IL-38 mRNA expression level of PBMCs from 30 SLE pa- tients and 20 healthy controls were determined by real-time quantitative reverse transcription poly- merase chain reaction （RT-PCR）. In addition, serum IL-38 levels of 72 SLE patients and 63 healthy controls were assessed by enzyme-linked immunosorbent assay （ELISA）. The nonparamet- rie Mann-Whitney U test or Chi-square test were used to compare the mRNA levels, serum levels and the detection rate of IL-38 between SLE patients and healthy controls. The Chi-square test or Student＇s t-test were used to compare the major clinical and laboratory parameters between pa- tients with detectable serum IL-38 （serum IL-38≥31.2 pg/mL） and patients with undeteetable serum IL-38 （serum IL-38 〈 31.2 pg/mL）. Results： The mRNA expression levels, detection rate and serum levels of IL-3g were significantly decreased in SLE patients compared to healthy controls （Z= -2.34, P=0.019; Z2 =10.19, P=0.002; Z= -4.04, P〈0.001）. Among patients with detectable serum IL-38, the anti-dsDNA antibody were all negative and their SLEDAI score was significantly decreased compared to those patients with undetectable serum IL-38 （t = -2.76, P =0. 020）. Conclusion： The impaired protective effect of IL-38 due to decreased pro-duction may participate in the onset and development of SLE.