苯并二氢吡喃腙衍生物舒张离体大鼠主动脉环

Vascular dilated effects of 2,3-2H-7-methoxy-4H-1-benzopyran-4-hydrazone derivatives on isolated aortic rings of rats

目的 观察苯并二氢吡喃腙衍生物对离体大鼠主动脉环的作用 .方法 取 SD大鼠胸主动脉环 ,采用离体血管张力实验法 ,用高钾引起血管收缩 ,药物孵育在前 ,溶剂对照在后 ,观察上述化合物对血管环张力的影响 .结果 以溶剂对照在 K+为 80 mmol· L-1 时的最大收缩幅度为 1 0 0 % ,其余取其收缩幅度相对值 . XY990 2 (1μmol· L-1 )在 K+为 65mmol· L-1时收缩幅度相对值明显低于对照 (47.9± 1 3.2 vs76.4± 1 5.3,P<0 .0 1 ) ,有拮抗作用 ;XY990 5(1μmol· L-1 )在 K+为 65 mmol· L-1 时收缩幅度相对值明显低于对照(37.8± 1 9.4 vs 66.8± 1 9.9,P<0 .0 1 ) ,有拮抗作用 ,XY990 1没有拮抗作用 .结论 苯并二氢吡喃腙衍生物 XY990 2、XY990

AIM To study the effect of 2,3 2H 7 methoxy 4H 1 benzopyran 4 hydrazone (MBH) derivatives on rat thoracic aorta rings in vitro . METHODS By isolated vascular methods, rings of thoracic aorta from SD rats with contraction by high K + were used to study the dilating effect of MBH derivatives, whose rings were incubated with the compound and then contrasted with DMSO solvent. RESULTS Data were expressed as 100% contraction of rings induce by K +(80 mmol·L -1 ) exposed to DMSO solvent (mean±SEM). The difference in contraction in rings exposed to XY9902 (1 μmol·L -1 )and DMSO solvent was significant (47.9±13.2 vs 76.4 ±15.3, P <0.01) for rings contracted by K +(65 mmol·L -1 ), which indicated antagonism of the compound XY9902. The same was true to the rings exposed to XY9905 (1 μmol·L -1 )(37.8±19.4 vs 66.8±19.9, P <0.01), which indicated antagonism of the compound XY9905. XY9901 did not show antagonism. CONCLUSION These data demonstrated that MBH derivatives XY9902 and XY9905 had vascular relaxation effect on rat aorta rings in vitro .