当归多糖对脑缺血再灌注损伤大鼠脑组织氧化应激水平及炎症因子表达的影响

Effect of Angelicae Sinensis Radix Polysaccharide on Oxidative Stress Level and Inflammatory Cytokine Expression of Brain Tissues in Rats with Cerebral Ischemia Reperfusion Injury

目的：探讨当归多糖对脑缺血再灌注损伤大鼠脑组织氧化应激及炎症因子表达的影响。方法：Wistar大鼠随机分为假手术组、脑缺血再灌注模型组、当归多糖低剂量组（30mg·kg^-1·d-1），当归多糖高剂量组（60mg·kg^-1·d^-1）及尼莫地平组（15mg·kg^-1·d^-1）；给药时间为术前15d，灌胃给药，每天1次，假手术组及模型组灌胃等体积的溶媒；末次给药结束后1h，采用线栓法制备脑缺血模型；在缺血2h后，拔出线栓实施24h再灌注；分别采用Bederon评分法、干湿重法、氯化三苯基四氮唑（TTC）染色法、分光光度法、放射免疫法及蛋白免疫印迹法检测当归多糖对神经功能学评分、脑组织含水量、梗死体积、氧化应激水平、炎症因子表达及Toll样受体-4（TLR-4）／核转录因子-KB（NF-KB）信号通路的影响。结果：与模型组比较，当归多糖低、高剂量组神经行为学评分，高剂量组脑组织含水量，低、高剂量组脑梗死体积比均显著降低（P〈0．05，P〈0．01）；与模型组比较，当归多糖低、高剂量脑组织超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH-Px）活性明显升高，而丙二醛（MDA）含量则显著降低（P〈0．05，P〈0．01）；与模型组比较，当归多糖高剂量组肿瘤坏死因子α（TNF-α）含量，低、高剂量组白细胞介素-1β（IL-1β）含量及TLR-4，NF-xBp65蛋白表达水平均显著性降低。结论：当归多糖具有保护大鼠脑缺血再灌注损伤的作用，该作用与其具有的抗氧化及抗炎作用有关。

Objective： To discuss the effect of Angelicae Sinensis Radix polysaccharide on oxidative stress level and inflammatory cytokine expression of brain tissues in rats with cerebral ischemia reperfusion injury. Method： Wistar rats were randomly divided into Sham group, ischemia reperfusion （I/R） group, ASRP groups （30, 60 mg·kg-l·d-1） and Nimodipine group （15 mg.kg-l·d-l）. On the 15th day before the operation, the drugs were intragastrically administered once a day. Line plug method was adopted to establish the rat model of cerebral ischemia reperfusion at 1 h after last administration. Neurological function scores, water content, infarction area, oxidative stress level, inflammatory cytokine expression and Toll like receptor-4 （TLR-4） /nuclear transcription factor-KB （NF-KB） signaling pathway were evaluated by Bederon scoring method, wet weight method,radioimmunoassay and Western blot, respectively. Result： Compared with model group, neurological function scores in low and high-dose ASRP groups, water content in high-dose ASRP group, and infarction area in low and high-dose ASRP groups were significantly decreased （P 〈 0. 05, P 〈 0. 01）. Compared with model group, activities of superoxide dismutase （SOD） and glutathion peroxidase （GSH-Px） were significantly elevated, and content of malondialdehyde （MDA） was significantly decreased in low and high-dose ASRP groups （P 〈 0.05, P 〈 0.01 ）. Compared with model group, tumor necrosis factor-a （TNF-a） content in high-dose ASRP group, and interleukin- 1/3 （IL-113） content and expressions of TLR-4 and NF-KB p65 were significantly decreased in low and high-dose ASRP groups. Conclusion： ASRP has a protective effect on ischemia reperfusion injury in rats, which may be correlated with the antioxidant and anti-inflammatory effects.