椰子壳挥发油抑制急性心肌损伤大鼠心肌细胞凋亡的分子机制

Molecular mechanism of coconut shell volatile oil inhibiting cardiomyocyte apoptosis in rats with acute myocardial injury

目的:探讨椰子壳挥发油抑制急性心肌损伤大鼠心肌细胞凋亡的分子机制。方法:选取Ⅱ导联心电图正常的60只Wistar大鼠,雌雄各半,随机分为6组:正常对照组,模型组,复方丹参滴丸组,挥发油高、中、低剂量组,每组10只,每日给药1次,连续7d。从实验的第6天开始,除正常对照组外,其它各组大鼠颈部皮下注射盐酸异丙肾上腺素20mg·kg-1·d-1,连续2d,建立急性心肌损伤模型。检测各组鼠心肌细胞凋亡指数、细胞凋亡基因Bcl-2和Bax的蛋白表达。结果:与正常对照组比较,模型组心肌细胞凋亡指数与Bax表达显著升高(P<0.01),Bcl-2表达显著降低(P<0.05);与模型组比较,挥发油高、中、低剂量组心肌细胞凋亡指数与Bax表达均显著降低(P<0.01),挥发油高、中剂量组Bcl-2表达显著升高(P<0.01)。结论:椰子壳挥发油通过上调心肌组织中Bcl-2的表达、下调Bax的表达来抑制细胞凋亡,从而发挥其保护心肌的作用。

Objective： To study the molecular mechanism of coconut shell volatile oil inhibiting cardiomyocyte apoptosis in rats with acute myocardial injury. Methods： Sixty half male and half female Wistar rats with normal II lead electrocardiogram were randomly divided into six groups： normal control group, model group, compound Danshen Dripping Pills group, volatile oil high, middle and low dose groups, with 10 rats in each group. The medicine was given 1 time a day, 7d consecutively. From the 6th day of the experiment, except the normal control group, 20mg.kgt.d-~ of isoproterenol hydrochloride was injected subcutaneously into the neck of other groups for 2 days to establish the acute myocardial injury model. The apoptotic index of myocardial cell, Bcl-2 and Bax protein expressions in the myocardium of rats were detected. Results： Compared with normal control group, the apoptotic index of myocardial cell and the expression of Bax in model group increased significantly （P〈0.01）, the expression of Bcl-2 decreased significantly （P〈0.05）; Compared with model group, the apoptotic index of myocardial cell and the expression of Bax in volatile oil high, middle and low dose groups were obviously reduced （P〈0.01）, the expression of Bcl-2 in volatile oil high, middle group were obviously increased （P〈0.01）. Conclusion： Volatile oil of coconut shell can protect the myocardium by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax in myocardium.