21日龄恐伤肾仔鼠情志与海马区L-DOPA、DA及DOPAC的相关性

Correlation study on emotional changes of 21-day-old neonatal rats of terror impairing kidney and L-DOPA,DA,DOPAC in hippocampus

目的:考察21日龄恐伤肾仔鼠情志与海马区左旋多巴(L-DOPA)、多巴胺(DA)及3,4-二羟基苯乙酸(DOPAC)的相关性。方法:通过旁观电击法建立恐伤肾孕鼠模型,通过对其21日龄仔鼠进行旷场试验、糖水偏好实验和悬尾实验,观察其情志改变;通过脑立体定位仪在右侧海马区采集脑透析液,应用HPLC-ECD法测定仔鼠脑透析液中L-DOPA、DA、DOPAC含量。结果:与对照组比较,模型组21日龄仔鼠垂直得分、水平得分均降低,糖水消耗量少、糖水偏好值低,在空中停止挣扎的时间较长(P<0.05);与对照组比较,模型组21日龄仔鼠海马组织细胞外液中各个灌流时间点L-DOPA、DA、DOPAC的水平均降低(P<0.05);21日龄仔鼠海马区DA、DOPAC与其垂直得分、水平得分、糖水偏好值呈正相关,与空中停止挣扎的时间呈负相关关系(P<0.05);神经递质L-DOPA与其糖水偏好值呈正相关,与空中停止挣扎的时间呈负相关关系(P<0.05)。结论:21日龄恐伤肾仔鼠情志与海马区L-DOPA、DA、DOPAC含量密切相关。

Objective： To study the correlation between emotional changes of the short-term effect and L-DOPA, DA, DOPAC in hippocampus of 21-day-old neonatal rats of terror impairing kidney. Methods： Pregnant rats model of terror impairing kidney was established by watching other rats shocked by electricity. Emotional changes were observed by open field test, sucrose preference test and tail suspension test on the 21st days after birth. Brain micro dialysis of the right hippocampus was collected with the stereotaxic instrument to determine the level of L-DOPA, DA, DOPAC by HPLC-ECD. Results： Compared with the control group, score of level and vertical movements, consumption of sucrose and sucrose preference value of the 21-day-old rats in the model group were reduced, while time to stop struggling in the air was increased, and the differences were statistically significant （P〈0.05）. Levels of DA, DOPAC, L-DOPA in the hippocampus of 21-day-old rats in the model group of cells were reduced in different liquid perfusion time points, and the differences were statistically significant （P〈0.05）. There was a positive correlation between the level of DA, DOPAC in hippocampus of the 21-day-old rats and score of level and vertical movements, and sucrose preference value, while there was a negative correlation between the level of DA, DOPAC and the time to stop struggling, and the differences were statistically significant （P〈0.05）. There was a positive correlation between the level of neurotransmitter L-DOPA and sucrose preference value,.while there was a negative correlation between the level of neurotransmitter L-DOPA and the time to stop struggling, and the differences were statistically significant （P〈0.05）. Conclusion： Correlation between emotional changes and L-DOPA, DA, DOPAC in hippocampus of 21-day-old neonatal rats of terror impairing kidney are closely related.