辰时针刺对自发性高血压大鼠辰时及酉时血压的不同影响

Observation on different influence of acupuncture during Chenshi on the blood pressure of spontaneously hypertensive rats during Chenshi and Youshi

目的:观察辰时针刺对自发性高血压大鼠(SHR)一天之中辰、酉两时辰血压的影响,同时总结辰时、酉时亚组治疗后2、4周时血压的变化规律。方法:24只11周龄雄性SHR随机分为针刺组和模型组,每组12只。另取12只WKY大鼠作为正常组。3组大鼠按时辰分为辰时(7:00-9:00)、酉时(17:00-19:00)两个亚组,每个亚组6只。针刺组于辰时给予电针干预足三里和曲池,留针15min;模型组与正常组在辰时接受捆绑刺激。分别在干预前,干预2、4周时,按两个亚组分别测量3组大鼠辰、酉两个时辰的血压。观察辰时、酉时亚组治疗前,治疗2、4周时两个时辰收缩压(SBP)、舒张压(DBP)变化,并分析3组大鼠总体血压的变化情况。结果:(1)就总体血压而言,在治疗2、4周时,针刺组的SBP和DBP均较模型组同期降低(P<0.05);与本组治疗前的SBP和DBP比较,正常组和模型组均无差异,针刺组在治疗2、4周时均降低(P<0.05);与治疗2周的SBP比较,针刺组在治疗4周时降低(P<0.05)。(2)就辰时血压而言,与治疗前的SBP比较,针刺组在治疗2、4周时均降低(P<0.05);(3)就酉时血压而言,与治疗前的SBP比较,针刺组在4周时降低(P<0.05);与2周的SBP比较,针刺组在4周时降低(P<0.05)。结论:辰时针刺在干预第2、4周时可以降低总体SBP和DBP;且辰时亚组在降低SBP的作用方面起效快,优于酉时亚组。

Objective： To observe the effects of acupuncturing during Chenshi on blood pressure of spontaneously hypertensive rats （SHR） during Chenshi and Youshi, and to summarize the change rule of blood pressure in Chenshi subgroup and Youshi subgroup after 2 and 4 weeks treatment respectively. Methods： Twenty-four 11-week old male SHR were randomly divided into acupuncture group and model group, with 12 rats in each group, The other 12 male WKY rats were selected as normal group. According to the time, rats in three groups were divided into Chert subgroup and You subgroup, with 6 rats in each group. Zusanli （ST36） and Quchi （LI11） of the acupuncture group received 15rain electro-acupuncture during Cbenshi. The model and normal group were bounded to stimulate. The changes of SBP and DBP were observed in all rats according to the subgroup in the corresponding period of time before, 2 and 4 weeks after intervention, and three group SHR＇s blood pressure changes were analyzed in overall. Results： ①In terms of overall blood pressure, the SBP and DBP in acupuncture group were both lower than those in model group 2 and 4 weeks after intervention （P〈0.05）. There was no statistical difference with SBP and DBP before and after treatment between normal group and model group. Two and 4 weeks after treatment, the SBP and DBP were both lower than those before treatment in acupuncture group （P〈0.05）. The SBP in acupuncture group 4 weeks after treatment was lower than that 2 weeks after treatment （P〈0.05）. ②In terms of blood pressure during Chenshi, the SBP 2 weeks and 4 weeks after treatment were both lower than those before treatment in acupuncture group （P〈0.05）. ③In terms of blood pressure duirng Youshi, the SBP in acupuncture group before treatment was no statistical different with that 2 weeks after treatment, but it was higher than that 4 weeks after treatment （P〈0.05）. The SBP 4 weeks after treatment was lower than that 2 weeks after treatment in acupuncture group （P〈0.05）. Conclusion： Acupuncture during Chenshi can reduce the overall SBP and DBP after 2 and 4 weeks treatment, and Chert subgroup has quicker effect on reducing SBP when compared with You subgroup.