大黄灵仙胶囊调控ABCB11和ABCC2干预胆结石形成的作用机制研究

Study on mechanism of Dahuanglingxian Capsule for intervening gallstone formation by regulating ABCB11 and ABCC2

目的探究大黄灵仙胶囊调控腺苷三磷酸结合盒转运子B亚族成员11(ABCB11)和腺苷三磷酸结合盒转运体C亚组2(ABCC2)表达水平干预胆结石形成的作用机制。方法将40只雄性C57BL/6小鼠分为正常组(N组)、模型组(M组)、熊去氧胆酸对照组(U组)和大黄灵仙胶囊治疗组(D组),每组10只。N组予普通饲料喂养,M组、U组和D组予致石饲料喂养8周,同时U组和D组分别予药物干预,1次/d,连续灌胃给药8周。造模成功后,分别采用荧光定量PCR和免疫组织化学法检测ABCB11、ABCC2的mRNA及蛋白表达水平。结果 M组小鼠ABCB11和ABCC2 mRNA和蛋白表达水平均较其余3组明显降低(P<0.00);而D组小鼠ABCB11和ABCC2 mRNA和蛋白表达水平与N组比较差异均无统计学意义(P>0.05)。结论大黄灵仙胶囊可通过调控ABCB11和ABCC2 mRNA和蛋白的表达预防胆结石的形成。

Objective To explore the mechanism of Dahuanglinxian Capsule for intervening gallstone formation by regulating the expression leveIs of ABCBll and ABCC2 mRNA and protein. Methods Forty male C57BL/6 mice were divided into the normal group（group N）, model group （group M）, ursodeoxycholic acid group （group U） and Dahuanglinxian Capsule treatment group （group D）, 10 cases in each group. The group N was fed with normal diet, while the group M, U and D were fed with lithogenic fodder for 8 weeks. Meanwhile the group U and D were given the medication intervention,once daily, for continuous 8 weeks of gavage. After successful modeling,mRNA and protein expression levels of ABCB11 and ABCC2 were detected by RT-PCR and immunohis- tochemistry. Results Compared with the other three groups,the expressions of ABCB11 and ABCC2 mRNA gene and protein in M group were significantly reduced（P〈0.01）;while, there was no statistical difference in the expressions of ABCB11 and ABCC2 mRNA and protein between the group D and N（P〉0.05）. Conclusion Dahuanglinxian Capsule can prevent the gallstone formation by regulating the expression of ABCB11 and ABCC2 mRNA and protein.