自噬抑制联合抗-VEGF对视网膜血管内皮细胞血管生成的作用

Role of combination of autophagy inhibition and anti-VEGF in angiogenesis of retinal vascular endothelial cells

目的观察自噬抑制联合抗-血管内皮生长因子(VEGF)对小鼠视网膜血管内皮细胞(RVECs)的增殖、迁移和管腔形成的作用。方法将RVECs随机分为4组:自噬抑制组、抗-VEGF组、自噬抑制+抗-VEGF联合组和对照组,4组细胞均在低氧条件下继续培养。分别采用Ed U法、Transwell法及Matrigel胶法检测细胞增殖、迁移和管腔形成。结果与对照组相比,其他3组的细胞增殖率、迁移细胞数和管腔形成数有不同程度的减少,且3-MA+抗-VEGF组的上述指标均明显小于3-MA组和抗-VEGF组。结论自噬抑制联合抗-VEGF在抑制视网膜新生血管中可能比单纯抗-VEGF发挥更好的作用。

Objective In this study,we explored the role of combination of autophagy inhibition and anti-VEGF in proliferation,migration and tube formation of mouse retinal vascular endothelial cells（RVECs）. Methods Well cultured mouse RVECs were randomly divided into four groups：autophagy inhibition group（add-ing autophagy inhibitor 3-MA）,anti-VEGF group（adding anti-VEGF-A neutralized antibody）,autophagy inhibi-tion＋anti-VEGF group（adding the two reagents）and the control group.All cells were then cultured in the hypoxic condition. The cell proliferation,migration and tube formation were detected by EdU,transwell and matrigel as-say,respectively. Results The cell proliferation rate,number of migrated cells and number of tube formation of the other three groups decreased when compared with the control group.These data above in autophagy inhibition＋anti-VEGF group were all significantly less than 3-MA group and anti-VEGF group. Conclusion Combination of autophagy inhibition and anti-VEGF may be more effective than simple anti-VEGF in inhibition of retinal neovascu-larization.