SASH1基因在非小细胞肺癌中表达的临床和预后意义

Clinical and prognostic significance of SASH1 expression in non-small cell lung cancer

目的:探讨候选抑癌基因SASH1(SAM-and SH3-domain containing 1)在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征和预后的关系。方法:应用免疫组织化学法检测202例NSCLC组织及110例癌旁组织中SASH1蛋白的表达,并分析与临床病理参数及预后的相关性。结果:(1)SASH1蛋白在癌组织阳性表达率为35.64%,显著低于癌旁组织的62.73%,差异具有统计学意义(P<0.001)。(2)SASH1蛋白阳性表达率远处转移组较非远处转移组明显降低,差异具有统计学意义(P=0.034),与肿瘤TNM分期相关(P=0.028)。(3)单因素Cox回归分析显示SASH1表达(P<0.001)、淋巴结转移(P=0.003)及TNM分期(P=0.001)与NSCLC的预后相关;多因素Cox回归分析显示SASH1表达(P<0.001)及TNM分期(P=0.026)是独立的预后指标。结论:SASH1基因在NSCLC中表达下调,该基因可能是NSCLC的抑癌基因,可能作为分子标记而用于NSCLC的诊断和治疗。

Objective：To investigate the expression of SASH1（SAM-and SH3-domain containing 1） in non-small cell lung cancer（NSCLC）,and to analyze the correlations of SASH1 expression with clinicopathological factors and prognosis in NSCLC.Methods：The expression of SASH1 was detected by immunohistochemistry in 202 NSCLC samples and 110 benign tumor-adjacent samples.STATA12.0 software was used for χ2 test,and SPSS20.0 software for Cox regression analysis and Kaplan-Meier survival analysis.Results：（1）The positive rate of SASH1 protein expression in NSCLC was significantly lower than that in adjacent noncancerous tissues（35.64% vs 62.73%,P0.001）.（2）SASH1 expression was significantly correlated with TNM stage（P=0.028） and distant metastasis（M）（P=0.034）.（3）Univariate analyses indicated that low SASH1 expression（P0.001）,lymph node metastasis（N）（P=0.003） and tumor TNM stage（P =0.001） were associated with the 5-year survival rate of NSCLC patients.Multivariate analyses further revealed that low SASH1 expression（P0.001） and TNM stage（P=0.026） were independent prognostic factors.Conclusion：SASH1 was significantly downregulated in NSCLC SASH1 could be used as an independent biomarker for the diagnosis and treatment of NSCLC.