HPLC波长切换法同时测定金嗓散结胶囊中的(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A

Simultaneous Determination of( R,S)-goitrin,Hydroxysafflor Yellow A,Salvianolic acid B,Baicalein-7-O-diglucoside and Baicalin-7-glucoside in Jinsang Sanjie Jiaonang by HPLC Wavelength Switching Method

目的:建立金嗓散结胶囊中(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A的同时测定方法。方法:采用HPLC法,应用Agilent HC-C18(250 mm×4.6 mm,5μm)色谱柱;以甲醇-乙腈(3∶1)与0.4%磷酸溶液为流动相,进行梯度洗脱;流速为1.0 m L·min^(-1);供试品采用50%甲醇超声提取。结果:(R,S)告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A的线性范围分别为4.150~83.00、5.120~102.4、11.07~221.4、4.590~91.80、8.140~162.8μg·m L^(-1),相关系数分别为0.999 2、0.999 9、0.999 4、0.999 7、0.999 5;5种成分分离良好,阴性对测定无干扰,平均加样回收率及相应的RSD分别为99.06%(1.27%)、99.54%(1.15%)、98.19%(0.89%)、97.88%(1.71%)、97.22%(0.99%)。结论:所建立的方法可用于金嗓散结胶囊的质量控制。

Objective：To develop a method for simultaneous determination of the content of （R,S）-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-O-diglucoside and baicalin-7-glucoside in Jinsang Sanjie Jiaonang.Methods：An HPLC method was adopted for the analysis,which was performed on a Agilent HC-C18（250 mm×4.6 mm,5.0 μm）chromatographic column with the mobile phase of methanol-acetonitrile（3∶1）（A）-0.4%phosphoric acid solution（B）in a gradient elution,the flow rate of 1.0 mL·min-1,and the samples were preprocessed with ultrasonic extraction in 50% methanol solution.Results：The linearity ranges of（R,S）-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-O-diglucoside and baicalin-7-glucoside were 4.150-83.00 μg·mL-1（r=0.999 2）,5.120-102.4 μg·mL-1（r=0.999 9）,11.07-221.4 μg·mL-1（r=0.999 4）,4.590-91.80 μg·mL-1（r=0.999 7）,8.140-162.8 μg·mL-1（r=0.999 5）,respectively.The five components were separated perfectly without disturbing peaks from negative samples.The average recoveries and the corresponding RSD were 99.06%（1.27%）,99.54%（1.15%）,98.19%（0.89%）,97.88%（1.71%）,97.22%（0.99%）,respectively.Conclusion：The method can be applied to the quality control of Jinsang Sanjie Jiaonang.