EMS诱变下水稻Hitomebore基因组内mPing转座子的转座

EMS Induced Transposition of mPing Transposons in Hitomebore Genome of Rice

mPing转座子是水稻中较常见的活跃转座子,组织培养、射线刺激、高压、激光辐射、逆境胁迫都可导致mPing转座子的激活。本研究利用甲基磺酸乙酯(EMS)诱变水稻品种一目惚(E10),在田间种植该诱导品种后,发现一株开花期明显提前11 d的突变体植株(E20),对其单株收获。第二年随机取E20种子50粒种植,获得了开花期明显提前10~13 d的突变体材料13株(E30a),其它35株(E30b)的开花期正常与E20相同,E30a与E30b株数比例为1:2.69,符合孟德尔遗传规律。利用29对引物对E30a进行mPing转座子检测,发现E30a中有14个转座子位点发生了跳出,且13株开花期提前转座子位点跳出情况一致,而非EMS处理对照(E10)植株未发生转座子跳出。因此,推测EMS诱导激活了E10中mPing转座子,E30a中14个转座子位点的跳出与突变体花期提前均为EMS诱导。为进一步深入探究mPing转座子跳出与水稻开花期提前的关系奠定基础。

m Ping transposon is a common active transposon in rice, which can activated by tissue culture, ray stimulation, high pressure, laser radiation and adversity stress. In this research we utilized EMS to induce Hitomebore（E10） variety of rice. After grown such an induced variety in the field, one mutant plant（E20） was found and reaped whose florescence was advanced by 11 days obviously. In the second year, 50 seeds of E20 were grown randomly and got 13 mutant plants（E30 a） whose florescence were advanced by 10-13 d. The florescence of the other 35 plants（E30 b） were normal, as same as E20. The ratio of E30 a and E30 b number was 1：2.69, which matched with the mendelian inheritance principle. We used 29 pairs of primers to test m Ping transposon of E30 a, and found 14 transposons sites of E30 a jumping out as same as the transposons sites of 13 plants florescence in advance, the non-EMS processing control（E10） plants had not the transposon jumping out. Therefore, it speculated that the EMS induction activated the m Ping transposon in E10. 14 transposons sites jumping out in E30 a and the advanced florescence of mutant were induced by EMS, which laid a foundation for further studying of the relationships between the jumping out of m Ping transposon and florescence of rice in advance.