猕猴桃SC-SSR遗传多样性分析及指纹图谱构建

Genetic Diversity Analysis and Fingerprints Construction of SC-SSR Markers in Kiwifruit(Actinidia Lind L.)

利用SC-SSR标记对32份猕猴桃种质材料进行了遗传多样性分析。从48对SC-SSR引物中筛选了11对引物进行PCR扩增,共扩增出200个条带,其中多态性条带194个,多态性比率为97.00%。各引物Nei's基因多样性指数(H)平均为0.272 6,Shannon's信息指数(I)平均为0.420 7。供试材料两两间的遗传相似系数(GS)在0.460 0~0.980 0之间,平均值为0.677 0,变幅为0.520 0,说明供试材料间存在较大的遗传差异。在遗传相似系数为0.70处可将32份猕猴桃材料分为3组:第一组中华猕猴桃+美味猕猴桃、第二组毛花猕猴桃、第三组软枣猕猴桃,聚类所得与猕猴桃传统分类基本一致。利用6对引物扩增的13个多态性位点构建了32份猕猴桃种质的DNA指纹图谱,可以将它们区分并准确鉴定。

SC-SSR was used to analyze the genetic diversity of 32 germplasms of Actinidia Lind L. PCR amplification was done on 11 pairs of primers that screened from 48 SC-SSR primers and 200 SC-SSR bands were obtained, including 194 polymorphic bands, with a polymorphism rate of 97.00%. The average Nei＇s gene diversity index（H） and Shannon＇s information index（I） were 0.272 6 and 0.420 7, respectively. The genetic similarity coefficient（GS） between two germplasms of 32 A. chinensis ranged from 0.460 0-0.980 0, with an average value of 0.677 0 and a range of 0.520 0, indicating that there was a huge genetic difference among these materials. The32 Actinidia germplasms were divided into three groups in the genetic similarity coefficient of 0.70： the first group were A. chinesis and A. delicosa, the second group was A. eriantha, the third group was A. arguta, and the constructed phylogenetic trees based on SC-SSR were consistent with traditional classification of the Actinidia. A total of 32 Actinidia DNA fingerprints were constructed from 13 polymorphic loci amplified by 6 pairs of primers,which could accurately distinguished and identified 32 Actinidia species materials.