长柱金花茶叶的化学成分研究(Ⅱ)

Chemical constituents from leaves of Camellia nitidissima var.longistyla(Ⅱ)

目的研究长柱金花茶Camellia nitidissima var.longistyla叶的化学成分。方法采用硅胶柱色谱、Sephadex LH-20、制备液相等多种手段进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果从长柱金花茶叶95%乙醇提取物中分离得到16个化合物,分别鉴定为山柰酚(1)、3,5,6,7,4′-五羟基二氢黄酮(2)、6-羟基山柰酚(3)、(-)-表阿福豆素(4)、(-)-表儿茶素(5)、山柰酚-3-O-β-D-葡萄糖苷(6)、α-菠菜甾醇(7)、β-胡萝卜苷(8)、5,7,3′,4′-四甲氧基黄酮(9)、7,3′,4′-三甲氧基-5-羟基黄酮(10)、5,7,3′,4′,5′-五甲氧基黄酮(11)、3-O-乙酰齐墩果酸(12)、正三十四烷醇(13)、β-香树脂醇(14)、α-菠菜甾醇-3-O-β-D-吡喃半乳糖苷(15)、芦丁(16)。结论所有化合物均为首次从该植物中分离得到,其中化合物2、3、9~11为首次从该属植物中分离得到。

Objective To investigate the chemical constituents in the root of Camellia nitidissima var.longistyla.Methods The chemical constituents were separated and purified by silica gel,sephadex LH-20 column chromatography,preparative HPLC and so on.Their structures were determined by physical constants and spectral analyses.Results Sixteen compounds were obtained and identified as kaempferol（1）,3,5,6,7,4′-pentahydroxyflavone（2）,6-hydroxykaempferol（3）,（-）-epiafzelechin（4）,（-）-epicatechin（5）,kaempferol-3-O-β-D-glucopyranoside（6）,α-spinasterol（7）,β-daucosterol（8）,5,7,3′,4′-tetramethoxyflavone（9）,7,3′,4′-trimethoxy-5-hydroxyflavone（10）,5,7,3′,4′,5′-pentamethoxyflavone（11）,3-O-acetyl oleanolic acid（12）,n-tetratriacontanol（13）,β-amyrin（14）,α-spinasterol-3-O-β-D-galactopyranoside（15）,and rutin（16）.Conclusion All compounds are isolated from the plant for the first time.Compounds 2,3,and 9—11 are isolated from the plants of Camellia for the first time.