复方血脂宁配伍对主要活性成分溶出变化规律的影响

Research on dissolution rule of main active ingredients in compatibility process of Compound Xuezhining

目的研究复方血脂宁在不同配伍条件下6种主要活性成分(橙黄决明素、红镰霉素-6-O-β-D-龙胆二糖苷、大黄素、二苯乙烯苷、荷叶碱及槲皮素)在水提液中的量变化,并采用Kohonen maps模型对成分量变化明显的中药进行数据分析,进而初步探究中药配伍对活性成分的溶出变化规律。方法对复方中的不同味药分别配伍,采用UPLC法研究6种主要活性成分在配伍前后的量变化;对数据进行分析,进一步选取配伍后量变化明显且趋势存在差异的荷叶作为研究对象,采用有监督的Kohonen maps模型分析不同配伍方式对荷叶中7种化学成分的影响。结果配伍后6种活性成分的量变化规律不同:荷叶碱、橙黄决明素、大黄素的量以降低为主,二苯乙烯苷的量降低,槲皮素的量升高,红镰霉素-6-O-β-D-龙胆二糖苷无明显规律;与山楂配伍对荷叶中7种化学成分的影响较大,趋势多为溶出增加。结论复方血脂宁不同配伍组合对活性成分溶出的影响不同,山楂与荷叶配伍后对荷叶所含成分溶出度的影响较大。

Objective To study the content change of six main active ingredients（orange diclofenac,rubrofusarin-6-O-β-Dgentiobioside,emodin,stilbene glucoside,nuciferine,and quercetin） in the aqueous extract under different compatibility conditions of Compound Xuezhining（CX）,using Kohonen maps model for data analysis of traditional Chinese medicine with obvious change in content,to further explore how the compatibility of TCM affect the dissolution characteristic of ingredient.Methods Through different combinations of different herbs in CX,the solubility of the six active ingredients was investigated by UPLC method before and after different compatibility modes; Analyzed the data and chosen Nelumbinis Folium which has significant quantity changes after compatibility as the object of research.The impacts of different compatibility modes on seven chemical compositions of Nelumbinis Folium were analyzed by supervised Kohonen maps model.Results The content change of six active ingredients after compatibility were different： the contents of nuciferine,orange diclofenac and emodin were mainly decreased,the content of stilbene glucoside was decreased,the content of quercetin was increased,and the content of rubrofusarin-6-O-β-D-gentiobioside showed no obvious regularity; When combined with Fructus Crataegi Pinnatifidae,seven chemical constituents in Nelumbinis Folium changed greatly and most of which were positively affected.Conclusion The effects of different compatibility modes on the solubility of active components are different in Chinese herbal formulae CX,and the content of chemical constituents in Nelumbinis Folium are more influential when combined with Fructus Crataegi Pinnatifidae.