基于外翻肠囊模型的黄芪-附子配伍对附子6种生物碱肠吸收的影响研究

Effect of Radix Astragali and Aconiti Lateralis Radix Preparata on intestinal absorption of six kinds of alkaloids in Aconiti Lateralis Radix Preparata

目的研究黄芪-附子药对配伍对附子3种单酯型生物碱(苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱)和3种双酯型生物碱(次乌头碱、新乌头碱、乌头碱)肠吸收的影响。方法运用大鼠外翻肠囊模型,选择十二指肠、空肠、回肠为研究肠段,以表观渗透系数(P_(app))为评价指标,考察黄芪对附子6种生物碱P_(app)的影响。结果当附子-黄芪3∶1时,黄芪在十二指肠和回肠能显著降低双酯型生物碱的P_(app),在3种肠段均能降低单酯型生物碱的P_(app);当附子-黄芪1∶1时,除次乌头碱在回肠外,黄芪能显著降低双酯型生物碱的P_(app);当附子-黄芪1∶3时,黄芪能显著降低单酯型生物碱(除回肠外)的P_(app),在各肠段均能显著降低3种双酯型生物碱的P_(app)。结论黄芪可抑制附子生物碱的吸收,且其抑制作用因配伍比例、生物碱的种类和肠段不同而不同。

Objective To study the effect of Radix Astragali and Aconiti Lateralis Radix Preparata on the intestinal absorption of the three kinds of monoester-type alkaloids（benzoylthioate,benzoyl neostearine,and benzoyl aconitine） and three kinds of diester-type alkaloids（hypaconitine,neaconitine,and aconitine） in Aconiti Lateralis Radix Preparata.Methods The rat duodenum,the jejunum,and the ileum were selected to study the intestinal segment.The apparent permeability coefficient（P_（app）） was used to evaluate the effect of Radix Astragali on the P_（app） of six kinds of alkaloids in Aconiti Lateralis Radix Preparata.Results When Aconiti Lateralis Radix Preparata and Radix Astragali was 3∶1,Radix Astragali can significantly reduce the dipeptide alkaloid P_（app） in the duodenum and ileum and reduce the monoester-type alkaloid P_（app） in three kinds of intestinal segments; When Aconiti Lateralis Radix Preparata and Radix Astragali was 1∶1,except in the ileum,Radix Astragali can significantly reduce the P_（app） of the diester-type alkaloid; When Aconiti Lateralis Radix Preparata and Radix Astragali was 1 ∶ 3,Radix Astragali can significantly reduce the P_（app） of the monoester-type alkaloid（except the parenteral） and significantly reduced the P_（app） of the three diester-type alkaloids.Conclusion Radix Astragali can inhibit the absorption of aconite alkaloids,and its inhibition effect is different due to different compatibility ratios,types of alkaloids and intestinal segments.