不同产地及生长年限曼地亚红豆杉中10-脱乙酰基巴卡亭Ⅲ、三尖杉宁碱、紫杉醇量的比较分析

Comparative analysis on contents of 10-deacetyl-bacratin Ⅲ,cephalomannine,taxol of Taxus madia from different habitats and growth years

目的分析和比较5个不同产地、不同生长年限曼地亚红豆杉中3种紫杉烷类活性成分量的差异。方法利用甲醇和三氯甲烷混合液超声浸提后再用甲醇溶解得到样品溶液;通过高效液相色谱法测定和比较10-脱乙酰基巴卡亭Ⅲ(10-DAB Ⅲ)、三尖杉宁碱和紫杉醇量及3者总量。结果不同产地、不同生长年限的曼地亚红豆杉中3种紫杉烷类及其总量间存在极显著性差异(P<0.01);10-DAB Ⅲ、三尖杉宁碱、紫杉醇和3种紫杉烷类总量相对较高的3个产地及生长年限分别依次为HK4>SM3>SM2、ZL3>HK4>ZL4、ZL3>SM2>HK4、HK4>ZL3>ZL6。除10-DAB Ⅲ与三尖杉宁碱量间不存在显著的相关性外,其余各成分间均存在极显著的正相关关系(P<0.01);不同产地及生长年限间紫杉醇与3种紫杉烷类总量间呈现出同升同降的趋势;聚类分析表明福建福州产区紫杉醇量具有一定的特殊性,而河南开封3种紫杉烷类总量有别于其他产区。结论产地和生长年限对曼地亚红豆杉中3种紫杉烷类量的变化具有明显的影响,可以通过对生物合成途径进行调控,继而达到获得更多紫杉醇原料药的目的。

Objective To analysis and compare the content differences of three effective components of taxanes of Taxus madia Rehd.from five different habitats and growth years by High Performance Liquid Chromatography（HPLC）.Methods The mixed liquor of CH3 OH and CHCl3 was used for the ultrasonic extraction of 10-deacetyl-bacratin Ⅲ（10-DAB Ⅲ）,cephalomannine and taxol before being dissolved by CH3 OH to obtain sample solution.By HPLC,the contents of three effective components were performed and compared.Results There were extremely significantly differences（P〈0.01） among the content of three effective components of taxanes of Taxus madia Rehd.from different habitats and growth years.Three different habitats and growth years of 10-DAB Ⅲ,cephalomannine,taxol,and taxanes that have relative higher total content were HK4 SM3 SM2,ZL3 HK4 ZL4,ZL3 SM2 HK4,HK4 ZL3 ZL6,respectively.Except for the contents of 10-DAB Ⅲ and cephalomannine,there were significant positive correlations among the contents of other components（P〈0.01）.The contents of taxol would rise or decline when the total contents of three kinds of taxanes rised or declined among different habitats and growth years.The clustering analysis results showed that the contents of taxol have certain peculiarity.In Fuzhou of Fujian,while the total contents of three kinds of taxanes were different from others in Kaifen of Henan.Conclusion Habitants and growth years have a significant impact on the contents change of three effective components of taxanes from Taxus madia Rehd..Therefore,regulation and control of biosynthetic pathway by choosing habitat conditions and harvest years should be considered,in order to obtain more crude drug of taxol in T.madia.