p(nBA-DEAEMA)的合成及其作为人工气管内表面修饰材料的初步研究

Preparation of p(nBA/DEAEMA) Copolymer Brushes and Its Feasibility Study as Surface Modification for Inner Face of Artificial Trachea

为制备p(nBA-DEAEMA)乳液及表面具有纤毛样结构的共聚物膜材料,采用半连续乳液聚合法合成p(nBA/DEAEMA)乳液,再用Velcro成型工艺将纯化干燥的产物制备成纤毛样膜.采用傅里叶红外光谱(Fourier Transform Infrared Spectrometer,FT-IR)、核磁共振光谱(Nuclear Magnetic Resonance Spectroscopy,~1 HNMR)、凝胶色谱(Gel Permeation Chromatography,GPC)、粒径分析、扫描电子显微镜(Scanning Electron Microscope,SEM)、静态接触角测定、示差扫描量热分析(Differential Scanning Calorimetry,DSC)及力学分析对材料进行详细表征.最后用浸提液实验对材料的生物安全性进行了初步评价.结果显示:FT-IR和~1 HNMR表明目标产物结构正确.共聚物乳液的多分散系数(polydispersity,PDI)为0.385,平均粒径(Z-average)为46.48nm.DSC结果显示共聚物的玻璃化温度(glass transition temperature,Tg)和粘流温度(viscous flow temperature,Tf)分别为33.2℃和47.7℃.纤毛样膜对水的接触角为102.3°,表面自由能13.81J·m2.SEM结果显示共聚物粒子大小为40~50nm,纤毛样层高度为5~10μm.细胞实验结果显示材料对大鼠气道上皮细胞(bronchial epithelial cells,BECs)具有可接受的生物安全性.

p（nBA/DEAEMA） nanoparticles of emulsion and polymeric cilia-like membranes were pre- pared. Two monomers of n-butyl acrylate （nBA） and 2-（diethylaminoethyl methacrylate） （DEAEMA） were polymerized by semi continuous emulsion polymerization method, and the cilia-like membranes were prepared by attaching a Velcro-like surface to partially coalesced films. Subsequently, the prepared materials were characterized by FT-IR,1HNMR, GPC, SEM, DSC combined with particle size analysis, static contact angle measurements and mechanical analysis. Finally,BECs from SD rats were used to char- acterize the biosecurity of the material. Correct structure of copolymer was also confirmed by FT-IR and HNMR. The particle size analysis of the copolymer emulsion was PDI 0.385 and Z-average was 46.48 nm, while Tg and Tf for copolymer was 33.2℃ and 47.7℃, respectively. The contact angle of polymeric films was 102.3% with water,and the surface free energy was 13.81 J·m2. Meanwhile,SEM photos showed the size of particles was 40 to 50 nm,and the height of cilia-like layer was about 5 to 10μm. The biosecurity of this material was eventually verified by cell experiments. The copolymer cilia-like membranes can be used as a surface modification material for artificial trachea.