摘要
以当年生半木质化枝条为外植体,对核桃楸组织培养技术及褐化的防止进行了研究。结果表明:采用0.1%HgCl_2对外植体灭菌10min,污染率和褐化率均相对较低,且生长最佳;选择以WPM为基本培养基,附加6-BA 2.0mg·L^(-1)、IBA 0.1mg·L^(-1)、Vc 300mg·L^(-1)的增殖效果最佳,能够有效防止褐化的发生,且腋芽生长良好。
Taking semi lignified branches as explants,control of browning for Juglans mandshurica and its tissue culture were studied.Result shows that the explants which were sterilized for 10 min by using 0.1%HgCl_2,their contamination & browning rate are relatively low,the growth is optimal;in WPM medium added 6-BA 2.0 mg·L^(-1),IBA 0.1 mg·L^(-1),Vc 300 mg·L^(-1) can obtain the optimal proliferation effect,which can effectively prevent the incidence of browning,grow of axillary bud being well.
出处
《防护林科技》
2017年第12期13-14,共2页
Protection Forest Science and Technology
关键词
核桃楸
组织培养
基本培养基
褐化
Juglans mandshurica
tissue culture
culture medium
browning
