江西省汉滩型汉坦病毒分离株AYW89-15全基因组序列分析

Complete genome sequence analysis of Hantaan virus strain AYW89-15 isolated in Jiangxi,China

目的了解江西省汉滩型汉坦病毒(Hantaan virus,HTNV)AYW89-15株的全基因组序列特征。方法设计和运用引物,采用RT-PCR方法分段扩增AYW89-15株的S、M、L全基因,对扩增产物进行克隆和序列测定。运用DNAstar和MEGA6.0软件分析基因序列。结果 AYW89-15株全基因组共有11 848个核苷酸,其中S、M、L基因分别含1 699、3 616、6 533个核苷酸,分别编码429、1 135、2 151个氨基酸。经序列比对,该病毒与国内外HTNV核苷酸同源性为79.7%~87.3%;氨基酸序列同源性为92.3%~98.4%。系统进化分析表明,AYW89-15株在HTNV系统进化树中独立分支。结论AYW89-15株为HTNV新的基因亚型,江西省存在新基因亚型的HTNV。

We investigated the complete genome characteristic of the Hantaan virus strain AYW89-15 isolated in Jiangxi Province,China.Primers were designed and were used to amplify the complete S,M and L segments by RT-PCR.The PCR product were then cloned and sequenced,the gene sequences were analyzed with DNAStar and MEGA6.0 software.Result showed that the complete gene sequence was 11 848 nucleotides in length,the S,M and L segments were 1 699 nt,3 616 nt and 6 533 nt respectively,encoding 429,1 133 and 2 151 amino acids respectively.The sequence identities between stain AYW89-15 and other Hantaan virus were 79.7%-87.3% at the nucleotide level and 92.3%-98.4% at the amino acid level.Phylogenetic analysis of HV showed AYW89-15 belonged to a new HTNV lineage.AYW89-15 was a new subtype of HTNV that exists in Jiangxi Province.