miRNA-30c在结直肠癌的表达与预后相关性分析

Expression and prognosis of miRNA-30c in colorectal carcinoma

目的探讨结直肠癌中miRNA-30c的表达与结直肠癌患者的临床病理及预后的相关性。方法采用实时定量PCR方法检测200对结直肠癌组织和邻近正常组织的miRNA-30c的表达水平,并对其与临床特征和预后的关系进行了统计分析。结果与正常的相邻组织相比,结直肠癌组织标本miRNA-30c的表达水平显著降低(P<0.001)。miRNA-30c的表达与肿瘤大小(P=0.012)、TMN分期(P=0.002)和淋巴结转移(P=0.004)呈正相关。单因素分析显示,miRNA-30c表达低的患者总生存明显短于表达水平较高的患者(P<0.001)。多因素分析显示miRNA-30c低表达可能是预后不良的独立预后因子(P<0.001)。结论 miRNA-30c可以用于预测结直肠癌患者的预后,并对选择合理的治疗措施有一定帮助。

Objective To investigate the expression level of miRNA30c in CRC, and analyze the correlation between miRNA30c expression level and prognosis. Methods 200 cases of patients with CRC were selected in ourhospital from January 2009 to January 2011. The expression level of miRNA30c in colorectal cancer and adjacentnormal tissues was detected by using Quantitative RTPCR, the association between miRNA30c expression andclinical characteristics and prognosis were statistically analyzed. Results Expression levels of miRNA30c weresignificantly lower in CRC tissues specimens compared with matched normal adjacent tissue （P〈0 001）. miRNA30c expression was positively correlated with tumor size （P = 0 012）, TMN stage （P = 0 002） and lymphnode metastasis （P = 0 004）. The univariate analysis showed that CRC patients with low miRNA30c had distinctly shorter overall survival （P〈0 001） than the patients with high miRNA30c expression level. The multivariate analysis was performed and indicated that low miRNA30c expression （P〈0 001） might be an independent prognostic predictor for poor prognosis. Conclusion miRNA30c is low expression in CRC tissues, and highexpression of miRNA30c contributes to the prognosis of CRC. miRNA30c can be used to predict the prognosisof colorectal cancer, and it is helpful to select reasonable treatment measures.