摘要
目的:探讨地塞米松对内毒素(ET)所致急性肺损伤(ALI)中血清血管紧张素转换酶(ACE)活性的影响,为临床ALI的药物选择提供理论依据。方法:将24只3个月龄雄性白兔随机分为生理盐水组(A组),ET组(B组),地塞米松+ET组(C组)。用静脉注射ET 700μg·kg^(-1)方法制作ALI模型,C组在ET注射前给予地塞米松(5 mg·kg^(-1))干预,在实验0、0.5、1、2、4 h行血氧饱和度(Pa O_2/Fi O_2)和血清ACE检测。结果:B组Pa O_2/Fi O_2在0.5 h降至最低为(265.4±70.2)mm Hg,随后逐渐升高,血清ACE活性在0.5 h升高至(68.39±6.29)u·m L^(-1),在1 h达到峰值(80.13±12.97)u·m L^(-1),2 h开始逐渐下降。C组Pa O_2/Fi O_2也呈现下降趋势,血清ACE活性呈现升高趋势,与B组比较具有显著性差异,与A组比较差异无统计学意义。结论:地塞米松可通过对血清ACE活性的抑制,对ET所致ALI产生一定的保护性作用。
Objective：To study the effects of dexamethasone on the changes of serumal angiotensin converting enzyme（ACE）activity in endotoxin（ET）-induced acute lung injury（ALI）and provide references for the choice of the ALI drugs in ckinic.Methods：The 24 rabbits were divided into three groups：saline control group（group A）,ET injuried group（group B）,dexamethasone＋endotoxin group（group C）.Rabbit model of ALI was induced by ET administered intravascularly with a dose of ET 700μg·kg-1.The rabbits in group C were injected with a dose of dexamethasone（5 mg·kg-1）before ET.The changes of blood oxygenation index（Pa O2/Fi O2）and serumal ACE activity were observed at 0 h,0.5 h,1 h,2 h,4 h respectively.Results：In group B,Pa O2/Fi O2 decreased significantly at 0.5 h（265.4±70.2 mm Hg）,then gradually increased.In group B,serumal ACE activity signifi cantly increased at 0.5 h（68.39±6.29 u·m L-1）,reached at peak at 1 h（80.13±12.97 u·m L-1）,gradually decreased after 2 h.In group C,the Pa O2/Fi O2 also decreased.The activities of serumal ACE were signifi cantly lower than those in group B.The Pa O2/Fi O2and the activity of serumal ACE were comparable to group A.Conclusion：Dexamethasone can possess some protective effects on ALI by inhibiting serumal ACE activity.
出处
《中国药物应用与监测》
CAS
2017年第6期340-343,共4页
Chinese Journal of Drug Application and Monitoring
基金
国家自然科学基金项目(81301995)
