学习经历对淡足侧沟茧蜂识别健康和感毒甜菜夜蛾幼虫的影响

Effects of learning experience of Microplitis pallidipes on the discrimination between healthy and NPV-infected Spodoptera exigua larvae

研究了淡足侧沟茧蜂2种学习经历对其识别健康和感毒甜菜夜蛾幼虫的影响。结果表明:寄生蜂幼虫期学习经历中,繁育寄主为未感毒幼虫的寄生蜂对健康和感毒幼虫的搜索时间、触角敲打时间、刺探次数、首选攻击率和寄生率均在感毒后84 h出现显著差异;而繁育寄主为感毒幼虫的寄生蜂,上述5个指标分别在感毒后84 h、72 h、72 h、72 h、84 h出现显著差异。寄生蜂成虫期学习经历中,在未感毒甜菜夜蛾幼虫体内产过卵的寄生蜂对健康和感毒幼虫的搜索时间、触角敲打时间、刺探次数、首选攻击率和寄生率分别在感毒后48 h、24 h、24 h、24 h、72 h出现显著差异;而对感毒幼虫有学习经历的寄生蜂,上述5个指标分别在感毒后84 h、84 h、72 h、84 h、84 h出现显著差异。研究结果可为侧沟茧蜂与核型多角体病毒的联合应用提供依据。

The effects of M. pallidipes two learning experiences on the discrimination between healthy and NPV-infected Spodoptera exigua larvae were investigated. The results indicated that in the learning experience of the parasitoids whose breeding hosts were healthy larvae, there were differences in the searching time, beating time of antenna, probing times, preferred attacking rate and parasitism rate at 84 h after treatment when the parasitoids discriminated between healthy and NPV-infected larvae,and there were differences in the five indices at 84 h,72 h,72 h,72 h and 84 h after treatment for the discrimination of the parasitoids whose breeding hosts were NPV-infected larvae; as to the learning experience of adult parasitoids on healthy larvae, there were differences in the five indices at 48 h,24 h,24 h,24 h and 72 h after treatment,while the learning experience of adult parasitoids on NPV-infected larvae led to the differences at 84 h, 84 h, 72 h, 84 h and 84 h after treatment. Our results revealed that the learning experience of M. pallidipes affected host discrimination ability,and these findings might provide basis for the combined application of M. pallidipes and NPV.