非线性动力学分析在重复经颅磁刺激对阿尔茨海默病大鼠学习记忆能力改善方面的应用

The Application of Nonlinear Dynamic Analysis on Repetitive Transcranial Magnetic Stimulation Improving the Learning and Memory of Alzheimer's Disease Rats

探讨重复经颅磁刺激(r TMS)对阿尔茨海默病(AD)大鼠学习记忆能力的影响,以及非线性动力学分析在大鼠脑电信号处理方面的应用。(1)将学习记忆无差异的60只SD大鼠随机分为(r TMS+AD)组、AD组和正常组,每组16只,多余的大鼠备用。(2)建立双侧海马区注射Aβ淀粉样蛋白(Aβ1-42)的AD大鼠模型,通过Morris水迷宫验证模型。(3)连续14 d对(r TMS+AD)组大鼠进行10 Hz的r TMS,AD组与正常组进行伪刺激。(4)利用水迷宫系统测试三组大鼠学习记忆能力,使用Cerebus 64通道神经电生理信号记录系统连续7 d采集三组大鼠头皮脑电。(5)在MATLAB中编程得到三组大鼠各个通道脑电信号能量谱以及LZ复杂度,利用SPSS软件对脑电信号和水迷宫数据进行统计分析。(1)AD组较正常组大鼠水迷宫逃避潜伏期变长,距离平台平均距离变远,差异有统计学意义(P<0.05);(r TMS+AD)组较AD组大鼠水迷宫逃避潜伏期变短,距离平台平均距离减小,差异有统计学意义(P<0.05)。(2)AD组较正常组大鼠脑电中低频(delta+theta)和高频(alpha+beta)的能量比值增高,部分通道差异无统计学意义(P>0.05);(r TMS+AD)组大鼠较AD组大鼠的低频与高频的能量比值部分通道升高部分降低,只有左颞叶的差异有统计学意义(P<0.05)。(3)(r TMS+AD)组较AD组大鼠各通道脑电LZ复杂度升高,AD组较正常组大鼠各通道脑电LZ复杂度降低,差异均有统计学意义(P<0.05)。r TMS能改善AD大鼠的学习记忆能力以及增加其脑电信号的LZ复杂度,LZ复杂度较AR模型功率谱估计更适合客观的诊断AD并且评价r TMS对AD大鼠脑电信号的影响。

To study the effects of repetitive transcranial magnetic stimulation （rTMS） on the learning adisease （AD） rats , and to discuss the application of nonlinear dynamic analysis in electroencephalograph （ EErats. （1）60 Sprague - Dawley（ SD） rats were randomly divided into group （ rTMS ＋ AD） , group AD ad control group , 16 rats in each group , and the remaining ones were used as spare rats. （2） To establish the AD rat model whose bilateral hippocampal area were in-jected with A bta amyloid protein （Ap1 -42） , and to evaluate the model using Morris water maze. （3） 10 Hz rTMS was conducted on the group （rTMS ＋AD） , and pseudo stimulation was done on the group AD and normal group for 14 d consecutively. （4） Morris water maze was used to test the learning and memory ability of rats in each group, and scalp EEG was electrophysiological signal recording system for 7 d consecutively. （5 ） The energy spectrum and LZ complexity of EEG signal in eachgroup were acquired by MATLAB, and using SPSS software for statistically analysing of EEGand water maze data. （1） The escape la-tency of group AD was longer than that of normal group, and the average distance of group AD from the platform was longer than that of normal group, and the difference was statistically significant （P 〈 0. 05） ; The escape latency of group （ rTMS ＋ AD） was shorter than that of the AD group, and the average distance of group （rTMS ＋ AD） from the platform was closer than that of the AD group, and the difference was statistically significant （P 〈0. 05） ; （2 ） The energy ratio of low frequency （ delta ＋ theta） and high frequency （ alpha ＋ beta） of group AD was higher than that of normal group. There was no statistical difference bet^veen the partial channels. The energy ratio of low frequency and high frequency of group （rTMS ＋ AD） was lower than that of group AD right temporal lobe and frontal lobe. In left parietal lobe and right parietal lobe, that was the opposite. Only the difference of left tem-poral lobe was statistically significant （ P 〈 0. 05 ）. （3） The LZ complexity of group （ rTMS ＋ AD） was higher than that of the AD group, the LZ complexity of group AD was lower than that of normal group, and the difference was statistically significant （P 〈0. 05）.rTMS can improve the learning and memory ability of AD rats and increase the LZ complesuitable for objectively diagnosing of AD and evaluating the effect of rTMS on EEG signal in based on AR model.