苏云金杆菌(Bacillus thuringiensis)与球孢白僵菌(Beauveria bassiana)复配对小菜蛾幼虫(Plutella xylostella)的影响研究

Effects of Compounds of Bacillus thuringiensis and Beauveria bassiana on Plutella xylostella larvae

研究不同浓度苏云金杆菌与球孢白僵菌复配混合剂对佳木斯地区小菜蛾幼虫死亡率的影响。用PBS缓冲液将苏云金杆菌与球孢白僵菌稀释成不同浓度梯度的菌悬液,采用正交分析法对苏云金杆菌及球孢白僵菌复配成7种不同浓度梯度的混合剂,并测定不同浓度混合剂对小菜蛾幼虫的死亡率,从而筛选出较适幼虫死亡的LD_(50)的比例及其浓度。经观察分析,在96 h内苏云金杆菌与球孢白僵菌复配为3:1时,小菜蛾幼虫的死亡率达到72%,其中在1×10^(-1)~1×10^(-6) g/mL浓度下幼虫死亡率较高。各混合液对小菜蛾幼虫致死效果不一,其中当苏云金杆菌与球孢白僵菌复配比例为3:1且浓度在1×10^(-1)~1×10^(-6) g/mL时,对昆虫幼虫致死效果明显。

Effects of mixed liquor with different concentrations of Bacillus thuringiensis and Beauveria bassianaon the mortality of Plutella xylostella larvae in Jiamusi were researched in this paper. PBS buffer was employed and the compound of B. thuringiensis and B. bassiana was diluted into different concentrations of suspension,the mixture of B. thuringiensis and B. bassiana was mixed into seven different concentration gradients by orthogonal analysis,and then the mortality of P. xylostella larvae at different concentrations was determined,and finally the more suitable larval LD_(50) proportion and concentration was screened. According to the observation and analysis,when the ratio of B. thuringiensis to B. bassiana was 3:1 in 96 h,the P. xylostella larvae mortality rate reached 72%,among them,the lethal rate of larvae in 10^(-1)~10^(-6) g/mL reached higher. The lethal effect of each mixture on the diamondback moth larvae was different. When the mixture ratio of Bacillus thuringiensis to Beauveria bassiana was 3:1 and the concentration was 10^(-1)~10^(-6) g/mL,the lethal effect on insect larvae was obvious.