地龙对矽肺模型大鼠α-平滑肌肌动蛋白和高迁移率族蛋白B1表达的影响

Experimental study on the expression of oL-SMA and HMGB1 in silicotic fibrosismodel rats interfered by lumbricus

目的观察地龙对矽肺模型大鼠α-平滑肌肌动蛋白（α-SMA）和高迁移率族蛋白B1（HMGB1）表达的影响。方法45只大鼠随机分为空白组、模型组和地龙干预组。建立大鼠矽肺模型，地龙干预组用地龙煎液4 ml/kg灌胃，空白组和模型组用等量生理盐水灌胃。每组在第7、14、28天处死5只大鼠。将肺组织行HE染色及天狼星红染色，用RT-PCR、Western blot法检测α-SMA和HMGB1mRNA和蛋白的表达。结果与空白组比较，模型组大鼠肺组织中α-SMA和HMGB1 mRNA及蛋白表达水平第7、14、28天时上调，差异有统计学意义（P〈0.01），与模型组比较，地龙干预组大鼠肺组织中α-SMA和HMGB1mRNA及蛋白表达水平在第7、14、28天时下调，差异有统计学意义（P〈0.05，P〈0.01）。结论地龙抑制矽肺胶原沉积，肺纤维化形成，可能与抑制肺组织HMGB1表达及α-SMA的活化相关。

Objective To observe the expression of alpha smooth muscle actin （α-SMA） and high mo-bility group protein B1 （HMGB1） in silicosis model rats interfered by lumbricus.Methods 45 rats were ran-domly divided into the control group, model group and group interfered by lumbricus. The silicosis model rats were established. The group interfered by lumbricus were intragastric administered with lumbricus decoction by the 4 ml/kg dose. The control group and model group were ig administered with the equal amount of normal saline. Each group were killed 5 rats on the 7th, 14th and 28th day. The lung tissues were stained with HE and Sirius red methods. The mRNA expressions of α-SMA and HMGB1 were determined with RT-PCR; The pro-tein levels of α-SMA and HMGB1 were determined with Western blotting.Results Compared with the control group, the expression levels of α-SMA and HMGB1mRNA and protein in lung tissue of model group were grad-ually increased in the 7^th, 14^th and 28^th days, the difference was statistically significant （P〈 0.01） . Compared with model group, the levels of α-SMA and HMGB1mRNA and protein in lung tissue of group interfered by lumbricus were gradually lowered in the 7th, 14th and 28th days, the difference was statistically significant （P〈0.05, P〈0.01） .Conclusion Lumbricus inhibits the collagen deposition and the formation of silicosis pulmo-nary fibrosis, which may be related to the inhibition of HMGB1 expression and activation of α-SMA in lung tis-sue.