PH-LHD大鼠模型中肥大细胞聚集与脱颗粒的规律和时间相关性研究

Study on the Regularity and Temporal Correlation of Mast Cell Aggregation and Degranulation in Rat Model of Pulmonary Hypertension in Left Heart Disease

该文探讨左心疾病所致的肺动脉高压(pulmonary hypertension in left heart disease,PHLHD)大鼠模型中肥大细胞(mast cell,MC)聚集与脱颗粒的规律和时间相关性。实验选取66只雄性未成年Sprague-Dawley(SD)大鼠,随机分对照组(6只)、假手术组(6只)和模型组(54只),对照组大鼠未做任何处理,当天即进入实验程序;假手术组大鼠行假手术后64 d进入实验程序;模型组大鼠通过实施升主动脉缩窄手术来制备左心疾病所致的肺动脉高压模型,模型组造模成功后分别在术后第1、3、5、8、15、22、36、50、64 d进入实验程序。该实验行肺组织的固定和切取并制作成病理切片,观察肺血管周边肥大细胞的聚集与脱颗粒。结果显示,20~50μm和50~100μm的肺血管周边,在第36 d时均发生明显的肥大细胞聚集现象,且以脱颗粒状态为主,并引起了整个肺组织肥大细胞密度的明显增加。>100μm的肺血管周边肥大细胞的聚集发生比较迟(第50 d),且以成颗粒状态的肥大细胞为主。在PH-LHD大鼠模型的形成过程中,肥大细胞起到了至关重要的作用。其中直径>100μm的肺肌性动脉周边的肥大细胞对肺高压形成的影响较小,而20~50μm和50~100μm的肺细血管周边的肥大细胞明显参与了肺高压的形成。

The article is to investigate the regularity and temporal correlation of mast cell aggregation and degranulation in rat model of pulmonary hypertension in left heart disease（PH-LHD）. 66 male minor SpragueDawley（SD） rats were randomly divided into control group（n=6）, sham operation group（n=6） and model group（n=54）. The rats in the control group entered the experimental procedure immediately without any treatment, and the rats in the sham operation group entered the experimental procedure after 64 days of sham operation, and the rats in model group entered the experimental procedure（6 rats each） on 1, 3, 5, 8, 15, 22, 36, 50 and 64 d, respectively, after the successful of banding of the ascending aorta. The lung tissue was fixed, cut and made into pathological sections to observe the aggregation and degranulation of mast cells peripheral pulmonary vessels. Obvious mast cell agregation was observed since 36 d in perivascular area of pulmonary arterioles with diameter 20-50 μm and 50-100 μm, and most cells were degranulated. The increased density of mast cells in whole lung tissue were observed. Mast cell agregation was observed since 50 d in perivascular area of pulmonary arterioles with diameter 100 μm, and most cells were granulated. Mast cells played a crucial role in PH-LHD rat model. The mast cells around the pulmonary arteries with diameter 100 μm had little effect on the formation of pulmonary hypertension, but the mast cells around 20-50 μm and 50-100 μm significantly involved.