猪RegIIIγ在小肠表达的特征及细胞类型

Expression Patterns and Cell Type of Porcine RegⅢγ in Small Intestine

RegⅢγ是一种新型的抗菌肽,主要在哺乳动物的肠道和皮肤表达,具有抗菌、抗炎、调节免疫反应和促进细胞增殖等生理功能。为了解猪RegⅢγ在肠道的表达特点,我们通过荧光定量PCR、蛋白质免疫印迹和免疫组化分别确定了猪RegⅢγmRNA和蛋白在仔猪肠道的表达水平以及在肠道表达的细胞类型。结果表明RegⅢγmRNA和蛋白质在十二指肠、空肠和回肠三个部位的表达水平无明显差异;除mRNA水平在断奶后有一个明显下降外,mRNA和蛋白质表达水平呈现随着周龄的增加而升高的趋势。免疫组化分析表明RegⅢγ蛋白主要在肠粘膜吸收性上皮细胞和Paneth细胞内表达。

RegⅢγ,a kind of novel antimicrobial protein with multiple functions,such as antibacterial,anti-inflammation,modulation of immune responses,and stimulation of cell proliferation,is highly expressed in intestine and skin of mammalian.To investigate the expression pattern and cell type,the mRNA and protein expression levels of RegⅢγ in neonatal piglets' intestine from 0-5 weeks were analyzed by real-time PCR and western bolt,and cells expressing RegⅢγ were determined by immunohistochemistry.PCR and western blot analysis showed that there were no significant differences of RegⅢγ mRNA and protein among the duodenum,jejunum,and ileum,and the mRNA and protein levels exhibit an increase trend with the development except for a decrease of mRNA after weaning.Immunohistochemistry assay revealed that RegⅢγ mainly expressed in absorption enterocytes and Paneth cells.