银杏叶提取物对慢性阻塞性肺疾病大鼠气道及肺血管重塑的影响

Effects of ginkgo biloba extract on airway and vascular remodeling in rat model of chronic obstructive pulmonary disease

目的探讨银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)大鼠气道及肺血管重塑的干预作用。方法将清洁级同龄雄性Wistar大鼠90只称重后,按照随机数字表法,随机分为A、B、C、D、E和F组,每组15只。B、C、D、E、F五组采用烟雾吸入、脂多糖气管内注入等进行COPD造模,C、D组分别在1~14 d和29~42 d腹腔内注射GBE(0.40 ml/kg/d),E、F组分别在1~14 d和29~42 d腹腔内注射红霉素(10 mg/kg2),43 d后对各组大鼠肺脏行病理学检测。结果 B、C、D、E、F五组均有COPD特征性改变,但程度不同。各组标准肺泡数的比较:A组与B、C、D、E、F组相比,差异均有统计学意义(P<0.05),B组与C、E两组相比较差异均有统计学意义(P<0.05)。各组平均肺泡面积的比较:A组与B、C、D、E及F组相比差异均有统计学意义(P<0.05);B组与C、E两组相比较差异均有统计学意义(P<0.05),C组与D、F两组相比较差异有统计学意义(P<0.05)。各组细支气管结构改变和肺泡内肺动脉的结构改变在统计学上意义上表现均相同:B组与A、C、D、E、F组相比较差异均有统计学意义(P<0.05),A、C、D、E、F各组之间比较差异均无统计学意义(P>0.05)。结论 GBE对COPD大鼠模型气道重塑及肺血管重塑有抑制作用,与红霉素组干预作用相类似。

Objective To study the intervention effect of ginkgo biloba extract( GBE) on airway and vascular remodeling in rat model of chronic obstructive pulmonary disease( COPD). Methods ninety wistar rats were randomly divided into group A,B,C,D,E and F. The rat model of COPD were established by exposure to cigarette smoke, intratracheally injection of lipopolysaccharide and the cold air stimulation composite factors in groups B,C,D,E and F five groups. Groups C and D were given intraperitoneally injection with GBE( 0. 4 ml/kg/d) from dayl to dayl 4 and day 29 to day 42. Groups E and F were given intraperitoneally injection with erythromycin( 10 mg/kg2) from dayl to dayl 4 and day 29 to day42. Forty-three days later,the rats were sacrificed for lung pathological examination. Results Group B,C,D,E and F all showed pathological changes characteristic of COPD to different extent. The comparison of standard number of alveolar of each groups: The differences between group A with B,C,D,E,F have statistical significance( P<0.05); The differences between Group B with C,E have statistical significance( P<0.05). The comparison of average alveolar area of each groups: The differences between group A with B,C,D,E,F have statistical significance( P<0.05); The differences between Group B with C,E have statistical significance( P < 0. 05);The differences between group C with D,F have statistical significance( P < 0. 05). The bronchioles structureand the pulmonary artery structure in alveoli changes of each groups have the same statistical significance: The differences between group A with B,C,D,E,F have statistical significance( P< 0.05); The differences of group C,D,E,F compare with each other have no statistical significance( P>0.05). Conclusion GBE has inhibitory effects on airway and vascular remodeling in rat model of COPD.