不同保存条件下呋喃丹及其代谢物在血液中的稳定性研究

Study on the stability of carbofuran and its metabolites in blood preserved at different conditions

目的研究呋喃丹及其代谢物呋喃酚在不同条件保存血液中的稳定性,为呋喃丹中毒案件的法医学鉴定提供实验依据。方法犬经口灌胃4LD50(13.5mg/kg)呋喃丹致死后,取血液分为五等份,分别为添加1%氟化钠(1%NaF)、添加2.5mg/m L枸橼酸钠(NC)、20℃、4℃和-20℃保存实验组。于保存当时(0d)、5d、7d、15d、40d、83d和150d取上述样品,多反应离子检测(MRM),气相色谱-质谱联用(GC-MS/MS)法检测其中呋喃丹及呋喃酚含量。结果血液中呋喃丹的含量随保存时间均呈下降趋势,7d显著下降(P<0.05),之后下降缓慢。不同条件保存血液中呋喃酚的含量均呈先升高后下降趋势。枸橼酸钠和氟化钠可加快血液中呋喃丹的分解。结论呋喃丹及其代谢物呋喃酚在保存检材中均可发生分解,20℃保存分解较快,4℃和-20℃保存分解速度较慢,不适宜用枸橼酸钠或氟化钠作抗凝剂或防腐剂。在呋喃丹的相关案件的法医学鉴定中,生物检材应注意冷藏、冷冻保存,并尽快送检。

Objective Study on the stability of carbofuran and its metabolite carbofuran phenol in blood preserved at different conditions,in order to provide a scientific evidence for forensic identification of carbofuran poisoning death.Methods The dogs were given intragastric administration with 4 LD50（13.5 mg/kg） of carbofuran,the blood were collected and divided into five equally groups preserved at 20℃（NC2.5 mg/m L）,20℃（1%Na F）,20℃,4℃ and-20℃,respectively.The concentrations of carbofuran and carbofuran phenol in above samples were detected by GC-MS/MS with MRM at 0 d、5 d、7 d、15 d、40 d、83 d and 150 d.Results The concentration of carbofuran in preserved blood were found to be significant decrease at 7 d（P 〈 0.05）,then a steady decline.In each condition,the concentration of carbofuran phenol in preserved blood showed an increasing trend firstly,then a declined tendency.The concentration of carbofuran and carbofuran phenol descending fast in blood at 20 ℃（NC） and 20 ℃（1%Na F）.Conclusion Carbofuran and carbofuran phenol in preserved specimens are found to be decomposed.The decomposition is quick at 20℃ and slow at-20℃.Citrate sodium and sodium fluoride are not suit for anticoagulation and antiputrefactiva.Biological specimens used for forensic identification of the carbofuran poisoning should be stored at refriferated or freezed,and be analyzed as soon as possible.