DNA一次和二次接触转移现象试验研究

Experimental study on primary transfer and secondary transfer of DNA

目的研究手部脱落细胞DNA能否通过握手、接触等方式发生一次转移和二次转移。方法寻找6名志愿者,在其洗手之后两两之间进行握手,然后让每一名志愿者接触已清洗的玻璃杯,最后对上述每一个玻璃杯进行DNA提取检测。结果在志愿者接触过的玻璃杯上,能够提取到因接触而发生一次转移和二次转移的基因座;同等条件下,一次转移获得的基因座数比二次转移获得的基因座数多。结论手部脱落细胞DNA能够发生接触转移现象,而且发生二次转移甚至多次转移是非常有可能的,因此在法医工作中,需要高度重视因DNA二次转移甚至多次转移而带来的污染问题。

Objective To study primary transfer and secondary transfer of exfoliated cells by handshaking and touching.Methods Firstly,volunteers wash hands,then shake hands between two volunteers,after that each volunteer touch the cleaned glass.Finally,pick up the DNA from the glass.Results STR loci that can be extracted from glasses.Under the same conditions,the number of STR loci obtained by primary transfer is more than that obtained by the secondary transfer.Conclusion Primary transfer and secondary transfer of exfoliated cells could happened by handshaking and touching.Therefore,in forensic work,we should pay great attention to the pollution problems caused by DNA transfer.