桑黄子实体与菌丝多糖抗肿瘤活性研究

Anti-tumor activity of polysaccharides from Phellinus igniarius fruiting body and mycelium

多糖是桑黄的主要生物活性物质之一,存在于桑黄子实体,发酵液以及其菌丝体中。本试验提取了桑黄子实体多糖和二种不同生长期的桑黄菌丝多糖,研究其分子结构,并且对其抗肿瘤活性进行了研究。试验结果表明,桑黄子实体多糖单糖组成为:半乳糖、葡萄糖、木糖和甘露糖;桑黄菌丝多糖的单糖组成为:半乳糖、葡萄糖和甘露糖,桑黄子实体多糖和桑黄菌丝多糖结构存在较大差异。桑黄子实体多糖对人肝癌细胞、人肺癌细胞和人宫颈癌细胞的抑制率IC_(50)值分别为:0.34 mg/mL、0.65 mg/mL、0.95mg/mL,表现出很强的抗肿瘤活性。体内和体外试验表明,桑黄子实体多糖抗肿瘤效果显著高于桑黄菌丝体多糖,不同生长期的菌丝多糖也呈现出不同的抗肿瘤活性,生长期长的菌丝多糖表现出更强的抗肿瘤活性。

Polysaccharides are the main bioactive components of Phellinus igniarius, it exists in Phellinus igniarius fruiting body, fermentation broth and mycelium of Phellinus igniarius. In this experiment, the extraction, molecular structure and anti-tumor activity of polysaccharides from fruiting body and two growth phase of mycelium were studied. The composition of polysaccharides monosaccharide of fruiting body are ~ galactose, glucose, xylose and mannoset polysaccharides monosaccharide composition of phellinus igniarius mycelial are ： galactose, glucose and mannose. Two polysaccharides had different structure. The anti-tumor experiments showed that the ICs0 values of inhibition rate of fruiting body polysaccharide on hepatoma carcinoma cell （HepG2）, lung cancer cell （A549）, hepatoma carcinoma cell （Hela）were 0.34 mg/mL, 0.65 mg/mL, 0.95mg/mL. The anti-tumor effect of Phellinus igniarius polysaccharide was significantly higher than that of mycelium polysaccharide ~ different growth period of polysaccharides mycelium had different anti-tumor activity, polysaccharides of mycelium within short growing period exhibited stronger anti-tumor activity. The experimental results provided an important theoretical basis for industrial of Phellinus igniarius in the future.