摘要
HL-60细胞是1株广泛应用于分化诱导研究的人早幼位白血病细胞株.在人肿瘤异种移植研究中,将白血病细胞成功地移植于裸鼠的报道很少,作者于1985年应用BALB/c裸鼠异种移植HL-60细胞系获得成功,建立了实体和腹水2种模型,现将结果报告如下. 材 料 与 方 法 一、人早幼粒白血病细胞株(HL-60)由美国Mount Sinai医学院引进,置含15％小牛血清的RPMI 1640培养基和含5％CO_2的培养箱培养.
In vivo models of HL-60 cells were successfully established as ascites and/ or solid forms in nude mice (BALB/c, nu/nu) . HL-60 ascites and solid tumor cells produced 100% lethal takes after their 7th transplant generation and were stabilized at serial transplant intervals of 14 and 40 days respectively. Morphological studies demonstrated that the ascites and solid tumor cells exhibited morphological characteristics similar to those of HL-60 cells in vitro. The establishment of transplantable HL-60 xenograft lines provides a potential tool for the screening of effective differentiation inducers.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第1期68-72,共5页
Acta Academiae Medicinae Sinicae
